Natural Spleen Cell Ligandome in Transporter Antigen Processing-Deficient Mice

Journal of Proteome Research
Elena LorenteDaniel López

Abstract

Peptides generated by proteases in the cytosol must be translocated to endoplasmic reticulum lumen by the transporter associated with antigen processing (TAP) prior to their assembly with major histocompatibility complex (MHC) class I molecules. Nonfunctional TAP complexes produce a drastic decrease of the MHC class I/peptide complexes presented on the cell surface. Previously, the cellular MHC class I ligandome from TAP-deficient cell lines was determined, but similar analysis from normal tissues remains incomplete. Using high-throughput mass spectrometry to analyze the MHC-bound peptide pools isolated from ex vivo spleen cells of TAP-deficient mice, we identified 210 TAP-independent ligands naturally presented by murine MHC class I molecules. This ligandome showed increased peptide lengths, presence of multiple nested set peptides, and low theoretical MHC binding affinity. The gene ontology enrichment analysis of parental proteins of this TAP-independent subligandome showed almost exclusively enrichment in tissue-specific biological processes related to the immune system as would be expected. Also, cellular components of the extracellular space (namely proteins outside the cell but still within the organism excluding the extr...Continue Reading

References

Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G J HämmerlingU Hämmerling
Dec 22, 1999·Immunogenetics·H RammenseeS Stevanović
Jan 13, 2000·Immunological Reviews·I A YorkK L Rock
Dec 25, 2002·Journal of Chromatography. a·Yasushi IshihamaMatthias Mann
Dec 24, 2005·Transfusion Medicine Reviews·Jan Simak, Monique P Gelderman
Sep 20, 2007·Nature Immunology·Peter E Jensen
May 1, 2008·European Journal of Immunology·Andreas O WeinzierlStefan Stevanović
Feb 19, 2009·Journal of Neuroscience Research·L SieE K Tan
Dec 30, 2009·The Journal of Experimental Medicine·Cláudia C OliveiraThorbald van Hall
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
Aug 18, 2012·Molecular Immunology·Margit H LampenThorbald van Hall
Nov 28, 2012·Molecular Immunology·Margarita Del ValLuis C Antón
Sep 24, 2013·Expert Review of Vaccines·Daniel LópezCarmen Mir
Jan 31, 2015·Molecular & Cellular Proteomics : MCP·Carolina JohnstoneDaniel López
Jun 23, 2015·Frontiers in Immunology·Cláudia C Oliveira, Thorbald van Hall
Jan 20, 2016·The Journal of Clinical Investigation·Elien M DoorduijnThorbald van Hall
Aug 8, 2018·Scientific Data·Heiko SchusterEtienne Caron
Aug 24, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nathalie VigneronRalf M Leonhardt

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Citations

Oct 6, 2020·F1000Research·Anita J ZaitouaMalini Raghavan

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