Natural transformation of Gallibacterium anatis.

Applied and Environmental Microbiology
Bodil M KristensenRosemary J Redfield

Abstract

Gallibacterium anatis is a pathogen of poultry. Very little is known about its genetics and pathogenesis. To enable the study of gene function in G. anatis, we have established methods for transformation and targeted mutagenesis. The genus Gallibacterium belongs to the Pasteurellaceae, a group with several naturally transformable members, including Haemophilus influenzae. Bioinformatics analysis identified G. anatis homologs of the H. influenzae competence genes, and natural competence was induced in G. anatis by the procedure established for H. influenzae: transfer from rich medium to the starvation medium M-IV. This procedure gave reproducibly high transformation frequencies with G. anatis chromosomal DNA and with linearized plasmid DNA carrying G. anatis sequences. Both DNA types integrated into the G. anatis chromosome by homologous recombination. Targeted mutagenesis gave transformation frequencies of >2 × 10(-4) transformants CFU(-1). Transformation was also efficient with circular plasmid containing no G. anatis DNA; this resulted in the establishment of a self-replicating plasmid. Nine diverse G. anatis strains were found to be naturally transformable by this procedure, suggesting that natural competence is common and t...Continue Reading

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K L Sisco, H O Smith
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M S Chandler
May 1, 1986·Molecular & General Genetics : MGG·J H Stuy, R B Walter
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·J J Zulty, G J Barcak
May 10, 2000·FEMS Microbiology Letters·D A IsraelM J Blaser
Oct 10, 2001·Journal of Bacteriology·S E Finkel, R Kolter
Jun 7, 2003·Journal of Clinical Microbiology·Anders Miki BojesenMagne Bisgaard
Jul 1, 1961·The Journal of General Physiology·S H GOODGAL, R M HERRIOTT
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Mar 17, 2005·Journal of Molecular Biology·Rosemary J RedfieldPaul R Langford
Oct 14, 2006·BMC Evolutionary Biology·Rosemary J RedfieldJohn He Nash
Oct 28, 2006·Nucleic Acids Research·Andrew D S Cameron, Rosemary J Redfield
Apr 23, 2008·Current Protocols in Bioinformatics·William ThompsonCharles E Lawrence
Nov 19, 2008·BMC Molecular Biology·Erin TracyRobert S Munson
Dec 18, 2008·Avian Pathology : Journal of the W.V.P.A·C NeubauerM Hess
Feb 26, 2009·Evolution; International Journal of Organic Evolution·Heather Maughan, Rosemary J Redfield
Aug 4, 2009·International Journal of Systematic and Evolutionary Microbiology·Peter KuhnertJoachim Frey
Aug 8, 2009·FEMS Microbiology Letters·Janine T BosséPaul R Langford
Aug 19, 2009·Molecular Phylogenetics and Evolution·Maria Pia Di BonaventuraPaul J Planet
Dec 4, 2009·Veterinary Research·Bodil Marie KristensenAnders Miki Bojesen
Jun 15, 2011·Veterinary Microbiology·Bodil M KristensenAnders M Bojesen

❮ Previous
Next ❯

Citations

Mar 20, 2013·Infection and Immunity·Ragnhild J BagerAnders M Bojesen
Mar 1, 2016·Frontiers in Microbiology·Christian J H von WintersdorffPetra F G Wolffs
Jun 13, 2015·Veterinary Research·Gry Persson, Anders M Bojesen
Apr 7, 2015·Journal of Avian Medicine and Surgery
Jun 15, 2011·Veterinary Microbiology·Bodil M KristensenAnders M Bojesen
Mar 5, 2017·Applied and Environmental Microbiology·MaFeng LiuAnChun Cheng
Feb 4, 2014·Journal of Bacteriology·Joshua Chang Mell, Rosemary J Redfield
Mar 28, 2018·Current Microbiology·Zhi-Feng PengChuan-Qing Wang
Mar 19, 2020·Emerging Infectious Diseases·Laura Van DriesscheFilip Boyen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.