Natural uranium disturbs mouse folliculogenesis in vivo and oocyte meiosis in vitro

Toxicology
Emilie ArnaultB Lefèvre

Abstract

We investigated whether uranium intoxication affects female fertility by assessing its effects on ovarian function and on the oocyte. We treated two groups of female mice for 15 weeks with 5, 50 or 400 mg/L of uranyl nitrate in drinking water. In the first group, mice were euthanized immediately after intoxication. Mice of the second group were paired after intoxication with untreated males. Dams and their female pups were euthanized 3 months after the end of intoxication. We assayed the kidneys, femurs and one ovary per female for U content and collected the other ovary for histology. The number and size of all the ovarian follicles were analyzed. Mice from the first group and female pups had significantly fewer large antral follicles (Ø > 200 microm) than the untreated mice. By contrast, dams in the second group had more secondary and early preantral follicles (Ø 70-110 microm) than untreated mice. However, U had no effect on follicle atresia. We then analyzed the in vitro effects of U on oocyte maturation and fragmentation. GV-oocytes were cultured in the presence of 1mM uranyl acetate and observed for 72 h. Oocyte maturation was slowed down by U during resumption of meiosis and at metaphase II. However, the rhythm and rate ...Continue Reading

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Citations

Jan 1, 2011·Reviews on Environmental Health·Doug Brugge, Virginia Buchner
Jul 19, 2011·Metallomics : Integrated Biometal Science·Erika MitchellBibudhendra Sarkar
Jan 26, 2011·Reproductive Toxicology·Gaëlle AngenardVirginie Rouiller-Fabre
Oct 18, 2008·Reproductive Toxicology·Alexandre FeugierMarie Claraz
Apr 3, 2014·BioMed Research International·Isabelle DublineauJocelyne Aigueperse
Jun 27, 2014·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Johan O NöthlingJan G Myburgh
Jul 20, 2018·International Journal of Environmental Health Research·Melanie C BuserHana R Pohl
Oct 18, 2019·Biological Trace Element Research·Shuang WangYuhui Hao

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