Natural yeast promoter variants reveal epistasis in the generation of transcriptional-mediated noise and its potential benefit in stressful conditions

Genome Biology and Evolution
Jian LiuJean-Pascal Capp

Abstract

The increase in phenotypic variability through gene expression noise is proposed to be an evolutionary strategy in selective environments. Differences in promoter-mediated noise between Saccharomyces cerevisiae strains could have been selected for thanks to the benefit conferred by gene expression heterogeneity in the stressful conditions, for instance, those experienced by industrial strains. Here, we used a genome-wide approach to identify promoters conferring high noise levels in the industrial wine strain EC1118. Many promoters of genes related to environmental factors were identified, some of them containing genetic variations compared with their counterpart in the laboratory strain S288c. Each variant of eight promoters has been fused to yeast-Enhanced Green Fluorescent Protein and integrated in the genome of both strains. Some industrial variants conferred higher expression associated, as expected, with lower noise, but other variants either increased or decreased expression without modifying variability, so that they might exhibit different levels of transcriptional-mediated noise at equal mean. At different induction conditions giving similar expression for both variants of the CUP1 promoter, we indeed observed higher ...Continue Reading

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Citations

Jul 25, 2015·FEMS Yeast Research·Souhir Marsit, Sylvie Dequin
Aug 21, 2018·ELife·Fabien DuveauPatricia J Wittkopp
Jan 27, 2019·Molecular Systems Biology·Sandra CortijoJames Cw Locke
May 28, 2019·Frontiers in Bioengineering and Biotechnology·Arun S RajkumarJohn P Morrissey
Oct 7, 2015·Frontiers in Genetics·Clément NielGhislain Rocheleau
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·M A EldarovA V Mardanov
Nov 17, 2020·Frontiers in Genetics·Konstantina GiannakouDaniela Delneri

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Methods Mentioned

BETA
environmental stresses
fluorescence-activated cell sorting
FACS
PCR
flow cytometry
Fluorescence
environmental stress

Software Mentioned

Cellquest
Lucy
Attune
Gene Ontology Term Finder
MultAlin
SAMtools
TraceTuner

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