Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection

Malaria Journal
Yang ChengEun-Taek Han

Abstract

Thirty-one glycosylphosphatidylinositol (GPI)-anchored proteins of Plasmodium vivax, merozoite surface protein 1 (MSP1), MSP1 paralogue, MSP4, MSP5, MSP8, and MSP10 have been reported from homologs of Plasmodium falciparum by gene annotation with bioinformatics tools. These GPI-anchored proteins contain two epidermal growth factor (EGF)-like domains at its C-terminus. Here, P. vivax merozoite surface protein 8 (PvMSP8) are considered as potential targets of protective immunity. Recombinant PvMSP8 (rPvMSP8) was expressed, purified, and used for the assessment of humoral and cellular immune responses in P. vivax-infected patients and immune mice. Moreover, the target epitope of ant-PvMSP8 antibodies and subcellular localization of PvMSP8 was also determined. The rPvMSP8 was successfully expressed and purified as soluble form as ~55 kDa. PvMSP8 was localized to the outer circle of pigments associated with the food vacuole. The rPvMSP8 protein had a high antigenicity (73.2% in sensitivity and 96.2% in specificity) in patients infected with P. vivax. IgG2 antibody subtype was the predominantly responses to this antigen. Antibody response to PvMSP8 increased up to day 7 and after that slightly decreased within a month. The longevity ...Continue Reading

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D E GoldbergG B Henderson
Jan 1, 1997·Annual Review of Microbiology·S E FrancisD E Goldberg
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
May 30, 2001·Molecular and Biochemical Parasitology·C G BlackR L Coppel
Oct 9, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert J HopkinsPhilip T Leese
Oct 27, 2004·Biochemical and Biophysical Research Communications·Oscar Perez-LealManuel A Patarroyo
Aug 30, 2005·Molecular and Biochemical Parasitology·Casilda G BlackRoss L Coppel
Feb 25, 2006·Cell·Alan F Cowman, Brendan S Crabb
Jul 19, 2006·Molecular and Biochemical Parasitology·Qifang ShiJames M Burns
Oct 10, 2008·Nature·Jane M CarltonClaire M Fraser-Liggett
Oct 25, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marthe C D'OmbrainLouis Schofield
Mar 6, 2010·Methods in Molecular Biology·Takafumi TsuboiYaeta Endo
Mar 15, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·M Andreína PachecoAnanias A Escalante
Oct 11, 2014·Nucleic Acids Research·Ivica LetunicPeer Bork

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Citations

Sep 11, 2018·PloS One·Yoelis Yepes-PérezManuel Alfonso Patarroyo
Jun 6, 2018·Frontiers in Cellular and Infection Microbiology·Carolina LópezManuel A Patarroyo
Apr 4, 2021·Pathogens·Elizabeth VillasisDionicia Gamboa

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Methods Mentioned

BETA
chips
peptide array
ELISA
flow cytometry

Software Mentioned

Simple Modular Architecture Research Tool ( SMART )
PASW Statistics
GraphPad Prism

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