PMID: 7523570Oct 1, 1994Paper

Naturally processed viral peptides recognized by cytotoxic T lymphocytes on cells chronically infected by human immunodeficiency virus type 1

The Journal of Experimental Medicine
T J TsomidesH N Eisen

Abstract

We have established long-term cultures of several cell lines stably and uniformly expressing human immunodeficiency virus type 1 (HIV-1) in order to (a) identify naturally processed HIV-1 peptides recognized by cytotoxic T lymphocytes (CTL) from HIV-1-seropositive individuals and (b) consider the hypothesis that naturally occurring epitope densities on HIV-infected cells may limit their lysis by CTL. Each of two A2-restricted CD8+ CTL specific for HIV-1 gag or reverse transcriptase (RT) recognized a single naturally processed HIV-1 peptide in trifluoroacetic acid (TFA) extracts of infected cells: gag 77-85 (SLYNTVATL) or RT 476-484 (ILKEPVHGV). Both processed peptides match the synthetic peptides that are optimally active in cytotoxicity assays and have the consensus motif described for A2-associated peptides. Their abundances were approximately 400 and approximately 12 molecules per infected Jurkat-A2 cell, respectively. Other synthetic HIV-1 peptides active at subnanomolar concentrations were not present in infected cells. Except for the antigen processing mutant line T2, HIV-infected HLA-A2+ cell lines were specifically lysed by both A2-restricted CTL, although infected Jurkat-A2 cells were lysed more poorly by RT-specific C...Continue Reading

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Citations

Jan 1, 1997·Springer Seminars in Immunopathology·M J Koziel, B D Walker
Oct 1, 2006·Journal of Biological Physics·Nicolas RapinOle Lund
Nov 8, 2011·Immunologic Research·Nadia Anikeeva, Yuri Sykulev
Apr 28, 2004·Microbes and Infection·Kathleen L Collins
Sep 16, 2003·Journal of Immunological Methods·Rachel Bras-GonçalvesJean-Gérard Guillet
Nov 15, 2002·Molecular Immunology·Carla A HerbertsCécile A C M van Els
May 2, 2002·Vaccine·Rob A GrutersAlbert D M E Osterhaus
Feb 22, 2003·Immunity·Kevin R BobbittKathleen L Collins
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Jan 10, 2001·The Journal of Experimental Medicine·P J GoulderC Brander
Jan 13, 2010·The Journal of Experimental Medicine·Anju BansalPaul A Goepfert
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Jul 28, 2010·Journal of Virology·Nicole M BeauchampMartha A Alexander-Miller

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