Nature of macrophage-T cell interaction in secondary helper cell generation in vitro. Genetic restriction of macrophage-T cell interaction, which determines T-B genetic restriction

European Journal of Immunology
P VogtM Feldmann

Abstract

To investigate the histocompatibility requirements for the macrophage-T cell interaction in the secondary antibody response, splenic T cells from antigen (carrier)-primed F1 hybrid mice were restimulated in vitro with carrier-pulsed F1, parental or allogeneic macrophages. Surviving T cell were cocultured with hapten-primed F1 or parental "B cells" and restimulated with the appropriate hapten-carrier conjugate. The IgG antibody-forming cell response was then measured using a plaque assay. Mapping of the genetic restriction was performed by use of different strain combinations. T helper cells could be restimulated in the presence of macrophages only provided they shared the I-A subregion of the major histocompatibility complex with the F1 T cells frm F1 hybrids restimulated with parental or I-A-identical macrophages were shown to only cooperate with parental B cells of the same I-A haplotype as the macrophages used for restimulation. The defect was at the level of the macrophage, as addition of macrophages of the I-A haplotype used for the restimulation culture reconstituted ability of F1 helper cells to cooperate with the I-A-nonidentical B cells.

References

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Citations

Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M H JuliusC L Sidman
Oct 1, 1982·Immunology Today·S Howie, W H McBride
Aug 1, 1990·Immunological Investigations·R M Chau, A S Lau
Jan 1, 1984·European Journal of Immunology·M S SandrinI F McKenzie
Aug 1, 1982·European Journal of Immunology·M H JuliusC L Sidman
Jul 1, 1989·Acta Oto-laryngologica·B Gloddek, J P Harris

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