PMID: 9435879Jan 1, 1997Paper

Nature of mutation in the human hprt gene following in vivo exposure to ionizing radiation of cesium-137

Environmental and Molecular Mutagenesis
A D da Cruz, B W Glickman

Abstract

The current study comprises the analysis of mutations in 10 individuals accidentally exposed to cesium-137 during the 1987 radiological accident in Goiânia, Brazil. Their exposures were among the highest experienced, ranging from 1 to 7 Gy. Peripheral T-lymphocyte samples were obtained 3.3 years after the original exposure and mutation was studied at the hprt locus using the 6-thioguanine-resistance selection assay. The mutational spectrum for the exposed population is comprised of 90 independent mutants. Based on T-cell receptor analysis, only 5% (5/95) were clonally related. Mutants were initially studied using RT-PCR and directly sequenced using an automated laser fluorescent DNA sequencer. Mutants that repeatedly failed to produce cDNAs were studied using a multiplex PCR assay with genomic DNA. Missense mutations were the most frequent event recovered, comprising 40% (23/57) of the spectral sample. An excess of events involving A:T base pairs was observed, exhibiting a significant difference (chi 2 = 12.7, P = 0.0004) when compared to the spontaneous spectrum. This finding may reflect the effect of ionizing radiation-induced damage, suggesting a potential similarity to radiation effects in prokaryotes. At the genomic level,...Continue Reading

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Citations

Jul 12, 2002·Environmental and Molecular Mutagenesis·Vasily N DobrovolskyRobert H Heflich
Jul 3, 2004·Cancer Research·Sederick C RiceBarry A Finette
Dec 1, 2000·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·D Averbeck
Mar 19, 2008·Mutation Research·Aparecido Divino da CruzMaria Paula Curado

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