PMID: 375980May 17, 1979Paper

Nature of the regions involved in the insertion of newly synthesized protein into the outer membrane of Escherichia coli

Biochimica Et Biophysica Acta
L de LeijB Witholt

Abstract

Outer membrane proteins are synthesized by cytoplasmic membrane-bound polysomes, and inserted at insertion sites which cover about 10% of the total outer membrane when cells grow with a generation time of 1 h. A membrane fraction enriched in outer membrane insertion regions was isolated and partly characterized. The rat at which newly inserted proteins are transferred from such insertion regions into the rest of the outer membrane was found to be very fast; the new protein content of insertion regions and that of the remaining outer membrane equilibrate completely within about 20 s at 25 degrees C. Given the rather rigid structure of the outer membrane and the multiple interactions between outer membrane components and the murein layer, lateral diffusion of newly inserted proteins from insertion sites to the remaining outer membrane is not likely to explain this rapid equilibration. Instead, the data support a model in which insertion regions move along the cell surface, leaving behind stationary, newly inserted outer membrane proteins.

References

Oct 31, 1975·Biochimica Et Biophysica Acta·V Braun
Jan 1, 1977·Annual Review of Biochemistry·C G Kurland
Feb 7, 1977·Biochemical and Biophysical Research Communications·A P Pugsley, P Reeves
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N LeeM Inouye
Mar 1, 1977·Journal of Bacteriology·K J Begg, W D Doanachie
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S InouyeM Inouye
Apr 18, 1977·Biochimica Et Biophysica Acta·R A Reithmeier, P D Bragg
Aug 8, 1977·Biochemical and Biophysical Research Communications·J SekizawaM Inouye
Nov 1, 1977·Biochimica Et Biophysica Acta·H van HeerikhuizenB Witholt
Nov 15, 1977·Biochimica Et Biophysica Acta·L De Leij, B Witholt
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·L Leive
Jan 1, 1978·Journal of Bacteriology·E T Palva, L L Randall
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W P SmithB D Davis
Apr 4, 1978·Biochimica Et Biophysica Acta·D Mug-Opstelten, B Witholt
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L L RandallL G Josefsson
May 16, 1978·European Journal of Biochemistry·S VarenneC Lazdunski
Sep 22, 1978·Biochimica Et Biophysica Acta·L de LeijB Witholt
Mar 17, 1977·Biochimica Et Biophysica Acta·B LugtenbergR Peters
May 2, 1977·European Journal of Biochemistry·L L Randall, S J Hardy
Jan 1, 1976·Annual Review of Microbiology·A R Glenn
Feb 1, 1975·Journal of Bacteriology·D H PopeJ V Landau
May 6, 1975·Biochimica Et Biophysica Acta·P OverathL Letellier
Feb 18, 1972·Science·S J Singer, G L Nicolson
Mar 25, 1973·Journal of Molecular Biology·F EngbaekO Maaloe
Jan 1, 1973·Annual Review of Biochemistry·B A Hamkalo, O L Miller
Nov 1, 1973·European Journal of Biochemistry·U HenningI Sonntag
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·I Haller, U Henning
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jan 15, 1963·Proceedings of the National Academy of Sciences of the United States of America·J R WARNERA RICH

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Citations

Jan 1, 1987·Progress in Biophysics and Molecular Biology·K BakerI B Holland
Nov 9, 2002·Biochimie·Zhenghua Cao, Phillip E Klebba
Jan 1, 1985·Annales De L'Institut Pasteur. Microbiology·J T Park, L G Burman
Dec 15, 2010·Seminars in Immunopathology·Can M UnalKristian Riesbeck
Jul 10, 1986·Biochimica Et Biophysica Acta·R M Price, D M Jacobs
May 30, 2016·Protein & Cell·Xuejun C Zhang, Lei Han
Jun 5, 1984·Journal of Molecular Biology·G H Vos-Scheperkeuter, B Witholt
May 15, 1981·European Journal of Biochemistry·J Wensink, B Witholt

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