Nature of the thymocytes associated with dendritic cells and macrophages in thymic rosettes

Cellular Immunology
K ShortmanR L Boyd

Abstract

Thymic rosettes, structures consisting of 3-30 thymic lymphoid cells attached to a central macrophage or dendritic cell, were released from mouse thymus tissue by collagenase digestion. They were shown to be preexistent structures within the thymus, but to be subject to extensive exchange with free thymocytes under certain conditions. An isolation procedure was developed, using a new technique of zonal unit-gravity elutriation, which minimized exchange and produced a completely pure sample of the larger rosettes. The rosette-associated thymocytes were analyzed by two- and three-color immunofluorescent staining and flow cytometry. The dominant cell type was a small, CD4+CD8+, cortical-type thymocyte. However, all of the established thymus subpopulations defined by CD4 and CD8, including CD4-CD8+ and CD4+CD8- mature thymocytes and CD4-CD8- early thymocytes, were also present in rosettes. Very few of the cells present were of an intermediate or transitional phenotype. Rosette-associated thymocytes were somewhat enriched in large dividing thymocytes, in CD4-CD8- thymocytes, and in mature thymocytes expressing the T-cell antigen receptor-CD3 complex. Their most striking characteristic was a marked depletion in small thymocytes lacki...Continue Reading

References

Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O LeoJ A Bluestone
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B A KyewskiH S Kaplan

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Citations

Sep 1, 1993·Immunology Today·R L BoydP Hugo
Jan 17, 2002·Journal of Immunological Methods·D H D GrayR L Boyd
Jun 1, 1997·Immunology and Cell Biology·K Ni, H C O'Neill
Dec 17, 2005·Immunity·Daniel H KaplanMark J Shlomchik
Sep 19, 2008·Immunity·María López-Bravo, Carlos Ardavín
Aug 30, 2011·Journal of Theoretical Biology·K WendelsdorfH T Banks
Feb 3, 2007·Molecular Immunology·Min ChenJin Wang
Jun 28, 2005·Seminars in Immunology·Cecilia Johansson, Brian L Kelsall
Feb 1, 1991·Immunology Today·R L Boyd, P Hugo
Jan 1, 1990·International Reviews of Immunology·K InabaM Inaba
Jan 1, 1990·International Reviews of Immunology·P J Fairchild, J M Austyn
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D VremecK Shortman

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