Nauclea officinalis inhibits inflammation in LPS-mediated RAW 264.7 macrophages by suppressing the NF-κB signaling pathway

Journal of Ethnopharmacology
Xiao-Ting ZhaiFen-Xia Zhu

Abstract

Nauclea officinalis has been traditionally used in China for the treatment of fever, pneumonia and enteritidis etc. This study aims to investigate effects of N. officinalis on the inflammatory response as well as the possible molecular mechanism in LPS-stimulated RAW 264.7 murine macrophage cells. Anti-inflammatory activity of N. officinalis (10, 20, 50, and 100µg/mL) was investigated by using LPS-induced RAW 264.7 macrophages. The NO production was determined by assaying nitrite in culture supernatants with the Griess reagent. The levels of TNF-α, IL-6 and IL-1β in culture media were measured with ELISA kits. Real time fluorescence quantitative PCR was detected for mRNA expression of iNOS, TNF-α, IL-6 and IL-1β. Western blot assay was performed to illustrate the inhibitory effects of N. officinalis on phosphorylation of IκB-α and NF-κB p65. Treatment with N. officinalis (10-100µg/mL) dose-dependently inhibited the production as well as mRNA expression of NO, TNF-α, IL-6 and IL-1β in RAW 264.7 macrophages. Western blot assay suggested that the mechanism of the anti-inflammatory effect was associated with the inhibition of phosphorylation of IκB-α and NF-κB p65. The results indicated that N. officinalis potentially inhibited the...Continue Reading

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Citations

Jul 15, 2016·Journal of Agricultural and Food Chemistry·Haichong WuGanzhen Deng
Nov 28, 2016·International Journal of Biological Macromolecules·Yuyan ChenRongmin Yu
Sep 29, 2020·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Guangren XuJianming Su
Nov 28, 2018·International Journal of Molecular Sciences·Ye Eun Jeong, Mi-Young Lee
Mar 25, 2019·International Journal of Environmental Research and Public Health·Ping-Kun TsaiShun-Fa Yang
Jan 8, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jian SunMin Huang
Apr 18, 2019·Journal of Agricultural and Food Chemistry·Yan-Li WuLi-Xia Chen
Jan 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fan ZhangYan Cao
Nov 27, 2021·Analytical Methods : Advancing Methods and Applications·Guanghou WangLixin Sun

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