eCovSens-Ultrasensitive Novel In-House Built Printed Circuit Board Based Electrochemical Device for Rapid Detection of nCovid-19

BioRxiv : the Preprint Server for Biology
S. MahariSonu Gandhi

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, also known as 2019-nCov or nCovid-19) outbreak has become a huge public health issue due to its rapid transmission making it a global pandemic. Currently, there are no vaccines or drugs available for nCovid-19, hence early detection is crucial to help and manage the outbreak. Here, we report an in-house built biosensor device (eCovSens) and compare it with a commercial potentiostat machine for the detection of nCovid-19 spike protein antigen (nCovid-19 Ag) in spiked saliva samples. A potentiostat based sensor was fabricated using fluorine doped tin oxide electrode (FTO) drop casted with gold nanoparticle (AuNPs) and immobilized with nCovid-19 monoclonal antibody (nCovid-19 Ab) to measure change in the electrical conductivity. Similarly, eCovSens was used to measure change in electrical conductivity by immobilizing nCovid-19 Ab on screen printed carbon electrode (SPCE). The performances of both sensors were recorded upon interaction of nCovid-19 Ab with its specific nCovid-19 Ag. Under optimum conditions, the FTO based immunosensor and proposed SPCE-based biosensor device displayed high sensitivity for early detection of nCovid-19 Ag, ranging from 1 fM to 1 uM. Our in-...Continue Reading

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