Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway

The Journal of Cell Biology
Hironori InabaMasaki Inagaki

Abstract

Primary cilia protrude from the surface of quiescent cells and disassemble at cell cycle reentry. We previously showed that ciliary reassembly is suppressed by trichoplein-mediated Aurora A activation pathway in growing cells. Here, we report that Ndel1, a well-known modulator of dynein activity, localizes at the subdistal appendage of the mother centriole, which nucleates a primary cilium. In the presence of serum, Ndel1 depletion reduces trichoplein at the mother centriole and induces unscheduled primary cilia formation, which is reverted by forced trichoplein expression or coknockdown of KCTD17 (an E3 ligase component protein for trichoplein). Serum starvation induced transient Ndel1 degradation, subsequent to the disappearance of trichoplein at the mother centriole. Forced expression of Ndel1 suppressed trichoplein degradation and axonemal microtubule extension during ciliogenesis, similar to trichoplein induction or KCTD17 knockdown. Most importantly, the proportion of ciliated and quiescent cells was increased in the kidney tubular epithelia of newborn Ndel1-hypomorphic mice. Thus, Ndel1 acts as a novel upstream regulator of the trichoplein-Aurora A pathway to inhibit primary cilia assembly.

References

Aug 1, 1995·The Journal of Cell Biology·B M Lange, K Gull
Oct 31, 2002·The Journal of Cell Biology·Alexander Dammermann, Andreas Merdes
Jan 28, 2004·Cell·Andrew W Murray
Feb 19, 2004·The Journal of Cell Biology·Yun LiangXueliang Zhu
Feb 26, 2005·Journal of Cell Science·Miwako NishizawaMasaki Inagaki
Jun 2, 2005·The Journal of Cell Biology·Lynne M Quarmby, Jeremy D K Parker
Aug 19, 2005·Molecular and Cellular Biology·Shinji SasakiShinji Hirotsune
Aug 5, 2006·Science·Veena Singla, Jeremy F Reiter
Jun 30, 2007·Current Biology : CB·Maïlys A S Vergnolle, Stephen S Taylor
Jul 3, 2007·Cell·Elena N PugachevaErica A Golemis
Oct 24, 2007·The Journal of Cell Biology·Susanne GraserErich A Nigg
Aug 30, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Charles T AndersonTim Stearns
Sep 12, 2008·The EMBO Journal·Masami YamadaShinji Hirotsune
Oct 8, 2008·The Journal of Cell Biology·Masahiko SugimotoMasaki Inagaki
Apr 7, 2009·Cell·Jantje M GerdesNicholas Katsanis
Nov 17, 2009·Cell·Erich A Nigg, Jordan W Raff
Feb 11, 2010·Journal of Cell Science·E Scott Seeley, Maxence V Nachury
Apr 17, 2010·Nature Reviews. Genetics·Sarah C Goetz, Kathryn V Anderson
Apr 30, 2010·Journal of Cell Science·Anahi Molla-HermanAlexandre Benmerah
Jul 21, 2010·Developmental Cell·Doris KinzelHeiko Lickert
Feb 2, 2011·The Journal of Cell Biology·Eliza ZyłkiewiczP Todd Stukenberg
Feb 2, 2011·PloS One·Mathieu ChansardMinh Dang Nguyen
Feb 18, 2011·Journal of Cell Science·Miho IbiMasaki Inagaki
Mar 12, 2011·Nature Cell Biology·Sehyun KimLeonidas Tsiokas
Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Hiroaki Ishikawa, Wallace F Marshall
Jun 17, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicholas J BradshawJ Kirsty Millar
Jun 28, 2011·European Journal of Cell Biology·Krysten J PalmerDavid J Stephens
Aug 5, 2011·Cell Cycle·Sehyun Kim, Leonidas Tsiokas
Sep 29, 2011·Cytoskeleton·Mathieu ChansardMinh Dang Nguyen
Feb 24, 2012·Molecular Biology of the Cell·Ping LiMasaki Inagaki
Apr 25, 2012·The Journal of Cell Biology·Akihito InokoMasaki Inagaki
May 25, 2012·Molecular Biology of the Cell·Olga V PlotnikovaErica A Golemis
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Hidemasa GotoMasaki Inagaki
Oct 5, 2013·Biomolecular Concepts·Nicholas J BradshawDinesh C Soares
Nov 6, 2013·The Journal of Cell Biology·Kazuhiro TateishiSachiko Tsukita
Oct 2, 2014·Nature Communications·Kousuke KasaharaMasaki Inagaki

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Citations

Jun 29, 2016·Nature Cell Biology·Irma Sánchez, Brian David Dynlacht
Sep 28, 2016·Cellular and Molecular Life Sciences : CMLS·Hidemasa GotoMasaki Inagaki
Oct 16, 2016·Cellular and Molecular Life Sciences : CMLS·Nicholas J Bradshaw, Mirian A F Hayashi
Apr 1, 2017·Cell and Tissue Research·Gerd Walz
Nov 17, 2017·BMC Cell Biology·Nicole DeVaulAnn O Sperry
Jul 18, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·A RitterJ Yuan
Apr 12, 2018·Cancer Biotherapy & Radiopharmaceuticals·Zhonghua WangJianping Cao
Aug 2, 2018·The Biochemical Journal·Mary MirvisW James Nelson
Dec 7, 2018·Journal of Cell Science·Pratim ChowdhuryRuhee Dere
Jan 10, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroka KashiharaSachiko Tsukita
Feb 24, 2018·Nature Communications·Kousuke KasaharaMasaki Inagaki
Feb 17, 2017·Biochemical Society Transactions·Vladislav KorobeynikovErica A Golemis
Apr 28, 2020·EMBO Reports·Andrea MartelloAndrea Caporali
Apr 30, 2020·The Journal of Cell Biology·Petra PejskovaLukas Cajanek
Jun 10, 2019·Cellular and Molecular Life Sciences : CMLS·Delowar HossainWilliam Y Tsang
Sep 25, 2020·The Biochemical Journal·Sarah E Conduit, Bart Vanhaesebroeck
Aug 23, 2020·International Journal of Molecular Sciences·Takashi ShiromizuYuhei Nishimura
Oct 16, 2019·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yuhei NishimuraMasaki Inagaki
Jan 16, 2019·Advanced Science·Yuhei NishimuraMasaki Inagaki
Aug 16, 2018·Science Signaling·Vladimir Joukov, Arcangela De Nicolo
Sep 19, 2018·Development·Lei Wang, Brian D Dynlacht
Mar 15, 2019·Oncology Letters·Michael HigginsTara McMorrow
Jan 3, 2019·Annual Review of Biochemistry·David K Breslow, Andrew J Holland
Jun 15, 2019·CNS Neuroscience & Therapeutics·Xinchen TengJ Marie Hardwick
Oct 28, 2020·Journal of Cellular Physiology·Bo WangPeijun Liu
Nov 19, 2020·Molecular Biology of the Cell·Marion FaillerBrian D Dynlacht
Dec 20, 2020·Diagnostics·Matteo SantoniCamillo Porta
May 19, 2021·Cell Communication and Signaling : CCS·Annapaola AngrisaniMarta Moretti
Jul 17, 2021·Neural Regeneration Research·Wen LiGuo-Hua Jin
Aug 26, 2021·Open Biology·Yuhei NishimuraMasaki Inagaki
Aug 24, 2021·Trends in Cell Biology·Kousuke Kasahara, Masaki Inagaki
Sep 9, 2021·Cell Reports·Gladiola Goranci-BuzhalaJay Gopalakrishnan
Nov 11, 2020·Developmental Cell·Océane Marescal, Iain M Cheeseman

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Methods Mentioned

BETA
two-hybrid
transfection
FACS
pull-down
ubiquitination
confocal microscopy
Assay
immunoprecipitation

Software Mentioned

softWoRx
JCB DataViewer
Photoshop Elements
Illustrator CS6
LSM browser
LSM
Adobe
ImageJ
Zen

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