NDF, a nucleosome-destabilizing factor that facilitates transcription through nucleosomes

Genes & Development
Jia FeiJames T Kadonaga

Abstract

Our understanding of transcription by RNA polymerase II (Pol II) is limited by our knowledge of the factors that mediate this critically important process. Here we describe the identification of NDF, a nucleosome-destabilizing factor that facilitates Pol II transcription in chromatin. NDF has a PWWP motif, interacts with nucleosomes near the dyad, destabilizes nucleosomes in an ATP-independent manner, and facilitates transcription by Pol II through nucleosomes in a purified and defined transcription system as well as in cell nuclei. Upon transcriptional induction, NDF is recruited to the transcribed regions of thousands of genes and colocalizes with a subset of H3K36me3-enriched regions. Notably, the recruitment of NDF to gene bodies is accompanied by an increase in the transcript levels of many of the NDF-enriched genes. In addition, the global loss of NDF results in a decrease in the RNA levels of many genes. In humans, NDF is present at high levels in all tested tissue types, is essential in stem cells, and is frequently overexpressed in breast cancer. These findings indicate that NDF is a nucleosome-destabilizing factor that is recruited to gene bodies during transcriptional activation and facilitates Pol II transcription t...Continue Reading

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Citations

Nov 27, 2019·Nature Communications·Christian AlbigCatherine Regnard
Sep 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jun XuDong Wang
Mar 15, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wei WangDong Wang
Apr 7, 2021·Current Opinion in Chemical Biology·Jinrong Min, Ke Liu
Aug 3, 2021·DNA Repair·Christina A GerodimosMichael R Lieber
Aug 14, 2019·Journal of Chemical Information and Modeling·Marco MontefioriGiorgio Colombo
Oct 31, 2021·Clinical Epigenetics·Chu XiaoJie He

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