NDM-1-producing Enterobacteriaceae in a teaching hospital in Shanghai, China: IncX3-type plasmids may contribute to the dissemination of blaNDM-1

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
Hongping QuJingyong Sun

Abstract

To provide the epidemiological dissemination and the genetic characteristics of bla(NDM-1) in a teaching hospital in Shanghai, China. Here, the carbapenemase genes of 114 CRE isolates were evaluated by polymerase chain reaction (PCR). Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE). Conjugation experiments and Southern blot hybridization were performed to determine the transferability of plasmids. Then plasmids were completely sequenced by the shotgun method. Two Klebsiella pneumoniae strains (RJA1227 and RJF866) and one Raoultella planticola strain (RJA274) were identified as NDM-1 positive. The two K. pneumoniae isolates belonged to ST11 and exhibited highly similar PFGE patterns. Shotgun sequencing showed that plasmid pRJF866 (ca. 110 kb) contained genes associated with the IncFII-FIB group and was highly similar to plasmid pKOX_NDM1. RJA274 (ca. 50 kb) harbored bla(NDM-1) on an IncX3 plasmid, which was nearly identical to plasmid pNDM-HN380 except that part of the ISAba125 element is missing. This is the first report of NDM-1-positive Enterobacteriaceae from Shanghai, China. IncX3 plasmids, reported in various species in the United Arab Emirates and China, may contribute to the dissemination of bl...Continue Reading

References

Dec 7, 2010·The Journal of Antimicrobial Chemotherapy·Yan QiYunsong Yu
Dec 30, 2010·Antimicrobial Agents and Chemotherapy·Yvonne PfeiferLaurent Poirel
Mar 15, 2011·The Journal of Antimicrobial Chemotherapy·Yan ChenYunsong Yu
Mar 23, 2011·Antimicrobial Agents and Chemotherapy·Bin LiYu-Xing Ni
Jun 10, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Neil GuptaAlexander J Kallen
Aug 24, 2011·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Oct 18, 2011·Emerging Infectious Diseases·Patrice NordmannLaurent Poirel
Nov 15, 2011·Trends in Microbiology·Patrice NordmannDavid M Livermore
May 19, 2012·The Journal of Antimicrobial Chemotherapy·Ying FuYunsong Yu
Mar 5, 2013·Diagnostic Microbiology and Infectious Disease·Longhua HuJingye Pan
Mar 19, 2013·Frontiers in Microbiology·Gopi Patel, Robert A Bonomo
Jun 12, 2013·Antimicrobial Agents and Chemotherapy·Tzu-Wen HuangShan-Chwen Chang

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Citations

Feb 21, 2016·Annals of Clinical Microbiology and Antimicrobials·Rui ZhengXueshan Xia
Mar 18, 2016·Emerging Infectious Diseases·Jun-Jie LiYohei Doi
Feb 3, 2016·Antimicrobial Agents and Chemotherapy·Charbel Al-BayssariJean-Marc Rolain
Apr 27, 2016·Antimicrobial Agents and Chemotherapy·Alexander M WailanSally R Partridge
Aug 24, 2017·Annals of Clinical Microbiology and Antimicrobials·Na DuYan Du
Oct 30, 2016·Frontiers in Cellular and Infection Microbiology·Jingnan LvFangyou Yu
Apr 13, 2018·Veterinary Sciences·Javier FernándezM Rosario Rodicio

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