NDRG1 activates VEGF-A-induced angiogenesis through PLCγ1/ERK signaling in mouse vascular endothelial cells.

Communications Biology
Kosuke WatariMayumi Ono

Abstract

Many diseases, including cancer, have been associated with impaired regulation of angiogenesis, of which vascular endothelial growth factor (VEGF)-A is a key regulator. Here, we test the contribution of N-myc downstream regulated gene 1 (NDRG1) to VEGF-A-induced angiogenesis in vascular endothelial cells (ECs). Ndrg1-/- mice exhibit impaired VEGF-A-induced angiogenesis in corneas. Tumor angiogenesis induced by cancer cells that express high levels of VEGF-A was also reduced in a mouse dorsal air sac assay. Furthermore, NDRG1 deficiency in ECs prevented angiogenic sprouting from the aorta and the activation of phospholipase Cγ1 (PLCγ1) and ERK1/2 by VEGF-A without affecting the expression and function of VEGFR2. Finally, we show that NDRG1 formed a complex with PLCγ1 through its phosphorylation sites, and the inhibition of PLCγ1 dramatically suppressed VEGF-A-induced angiogenesis in the mouse cornea, suggesting an essential role of NDRG1 in VEGF-A-induced angiogenesis through PLCγ1 signaling.

References

Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·Q S JiG Carpenter
Jan 1, 1997·Annual Review of Biochemistry·W D SingerP C Sternweis
Jun 22, 2000·Biochemical and Biophysical Research Communications·K L AgarwalaT Miyata
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Oct 16, 2001·American Journal of Physiology. Cell Physiology·A P McLaughlin, G W De Vries
May 27, 2003·International Journal of Cancer. Journal International Du Cancer·Katsuaki MasudaMichihiko Kuwano
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Jun 14, 2003·Biochemical and Biophysical Research Communications·Yoshitaka TaketomiIchiro Kudo
Apr 15, 2004·Molecular and Cellular Biology·Tomohiko OkudaToshiyuki Miyata
Nov 30, 2005·The Journal of Biological Chemistry·Baosheng ChenYoel Sadovsky
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Anna-Karin OlssonLena Claesson-Welsh
Sep 11, 2007·Cellular Signalling·Jing FangBing-Hua Jiang
Sep 13, 2008·Pflügers Archiv : European journal of physiology·S K InglisS M Wilson
Sep 25, 2009·Leukemia Research·Mario P TschanMartin F Fey
Nov 4, 2009·Traffic·Alexander F BrunsSreenivasan Ponnambalam
Aug 6, 2010·Blood·Bao HoangAlan Lichtenstein
Apr 7, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Koichi AzumaMichihiko Kuwano
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Sina Koch, Lena Claesson-Welsh
Oct 16, 2012·Advances in Biological Regulation·Hyun-Jun JangPann-Ghill Suh
Jan 24, 2013·The Journal of Biological Chemistry·Morgane GourlaouenAndrew R Reynolds
Sep 18, 2013·Advances in Biological Regulation·Yong Ryoul YangPann-Ghill Suh
Aug 8, 2015·Journal of Cellular Physiology·Francesco Moccia, Germano Guerra
Jul 28, 2016·Nature Reviews. Molecular Cell Biology·Michael SimonsLena Claesson-Welsh

❮ Previous
Next ❯

Methods Mentioned

BETA
pulldown
PMA
hECs
co-immunoprecipitation
transfection
proximity ligation assay
flow cytometry
Co-IP
PCR
fluorescence imaging

Software Mentioned

CellQuest Pro
ImageJ

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.