NDRG1 regulates osteosarcoma cells via mediating the mitochondrial function and CSCs differentiation.

Journal of Orthopaedic Surgery and Research
Tong ZhaoWenji Wang

Abstract

Cancer stem cells (CSCs) are mainly contributed to malignancy metastatic potential and resistant therapy of osteosarcoma (OS). The mitochondria-related apoptosis was generally accepted as the target of tumor therapy. However, the effect of N-myc downstream-regulated gene 1 (NDRG1) on CSCs and mitochondrial health in OS is still unknown. In OS cells, MG63 and U2OS, the siRNA of NDRG1 were conducted. Transwell, western blot, RT-qPCR, and mitochondria isolation were used to identify the effect of NDRG on OS cells and mitochondria. Moreover, the differentiation-related factors of CSCs were determined. After downregulation of NDRG1, the cell viability, invasion ability decreased whereas cell apoptosis increased. The expressions profiles of fibronectin, vimentin, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP) 2, MMP9, and MMP13 were downregulated, but E-cadherin expression level was upregulated by NDRG1 siRNA. At the same time, cytochrome (Cyt) C levels were increased in cytosol with the decreasing in mitochondria after siRNA treatment. The mitochondrial membrane potential (MMPs) was declined, and the function of mitochondria was impeded. The expressions of uncoupling proteins (UCP) 2, voltage dependent ani...Continue Reading

References

Aug 22, 2006·Carcinogenesis·Zaklina Kovacevic, Des R Richardson
Jul 14, 2009·The Journal of Biological Chemistry·Stephen H SchillingMichael B Datto
Jul 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Veerle MelotteManon van Engeland
Jan 23, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jan SkodaRenata Veselska
Jul 24, 2018·Biochimica Et Biophysica Acta. General Subjects·Nathalie VaesVeerle Melotte
Sep 19, 2018·Molecular and Cellular Biology·Ana AndjelkovićHoward T Jacobs
Jan 24, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bekesho GeletaZaklina Kovacevic

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