NDRG2 is a new HIF-1 target gene necessary for hypoxia-induced apoptosis in A549 cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Lifeng WangXinping Liu

Abstract

The NDRG2 gene belongs to a family of N-Myc downstream-regulated genes (NDRGs) and is expressed in many normal tissues. NDRG2 gene expression has been shown to be regulated in the stress response of certain cells. However, its function is not yet fully understood. Many studies have demonstrated that hypoxia, one of the stress responses, induced apoptosis in several cell types. In the current study, we investigated NDRG2 involvement in hypoxia response and found that NDRG2 expression was markedly up-regulated in several tumor cell lines exposed to hypoxic conditions or similar stresses at the mRNA and protein level. We also observed that the expression of NDRG2 was regulated by Hypoxia-inducible factor 1 (HIF-1) in tumor cells under hypoxia. Three hypoxia-responsive elements (HREs) in the NDRG2 promoter were identified. HRE1 could directly bind Hif-1 in vivo. Importantly, we found that silencing or enforcing the expression of NDRG2 could strongly inhibit or increase apoptosis. In addition, our data also showed that Ndrg2 was able to be translocated from the cytoplasm to the nucleus, and the segment from 101 to 178 amino acids of Ndrg2 is responsible for its translocation. Taken together, this study suggests that NDRG2 is a Hif-1...Continue Reading

References

Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
Feb 1, 1997·Kidney International·I KvietikovaM Gassmann
Dec 28, 1999·Annual Review of Cell and Developmental Biology·G L Semenza
Feb 22, 2001·Journal of the National Cancer Institute·M Höckel, P Vaupel
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hua SuYuet Wai Kan
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Roland H Wenger
Sep 19, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Frank RoseJörg Hänze
Jan 18, 2003·Experimental Cell Research·Megan Fabbro, Beric R Henderson
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Yanchun DengMarie Chia-mi Lin
Dec 19, 2003·Biochemical and Biophysical Research Communications·Jörg HänzeFrank Rose
Jan 13, 2004·Journal of Applied Physiology·Karen A Seta, David E Millhorn
Jun 23, 2004·Neurobiology of Disease·Cathy MitchelmoreNiels A Jensen
Aug 24, 2004·The Journal of Biological Chemistry·Utta Berchner-PfannschmidtJoachim Fandrey
Nov 5, 2004·World Journal of Gastroenterology : WJG·Xiao-Lan HuLi-Bo Yao
Nov 30, 2005·The Journal of Biological Chemistry·Baosheng ChenYoel Sadovsky

❮ Previous
Next ❯

Citations

Jan 5, 2011·Histochemistry and Cell Biology·Zhongchan SunHaichang Wang
Dec 7, 2011·Journal of Molecular Histology·Lijuan LiuYanchun Deng
Jan 12, 2013·Medical Oncology·Shu-Jun LiLibo Yao
Feb 27, 2014·Nature Communications·Shingo NakahataKazuhiro Morishita
Aug 12, 2008·Nucleic Acids Research·Na LiuLibo Yao
Aug 3, 2010·Journal of Experimental & Clinical Cancer Research : CR·Jian-Jun MaTing-Yi Bao
May 7, 2014·Brain Research·Yu-Long MaLi-Ze Xiong
Feb 4, 2010·Cellular and Molecular Life Sciences : CMLS·Lan ShenLibo Yao
Feb 11, 2009·The Journal of Physiology·Victoria C FolettaKen R Walder
Jun 16, 2011·Neurochemistry International·Toshiaki TakeichiOsamu Hori
Mar 9, 2010·Experimental Cell Research·Junye LiuGuozhen Guo
Sep 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jiandong YangKefeng Dou
Mar 25, 2010·Cancer Science·Hai ShiQingchuan Zhao
Jan 5, 2010·Biochemical and Biophysical Research Communications·Hiroshi FurutaKazuhiro Morishita
Sep 6, 2015·Acta Biochimica Et Biophysica Sinica·Kaifeng LinYan Li
Dec 3, 2014·Asian Pacific Journal of Tropical Medicine·Sheng QiangMin Huang
Apr 27, 2016·Apoptosis : an International Journal on Programmed Cell Death·Wei HuJianyuan Gao
Apr 6, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Yurong TaoLibo Yao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis