NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells.

Immune Network
Kyeongah KangJong-Seok Lim

Abstract

N-myc downstream-regulated gene 2 (NDRG2), a member of a newly described family of differentiation-related genes, has been characterized as a regulator of dendritic cells. However, the role of NDRG2 on the expression and activation of transcription factors in blood cells remains poorly understood. In this study, we investigated the effects of NDRG2 overexpression on GATA-1 expression in PMA-stimulated U937 cells. We generated NDRG2-overexpressing U937 cell line (U937-NDRG2) and treated the cells with PMA to investigate the role of NDRG2 on GATA-1 expression. NDRG2 overexpression in U937 cells significantly induced GATA-1 expression in response to PMA stimulation. Interestingly, JAK2/STAT and BMP-4/Smad pathways associated with the induction of GATA-1 were activated in PMA-stimulated U937-NDRG2 cells. We found that the inhibition of JAK2 activation, but not of BMP-4/Smad signaling, can elicit a decrease of PMA-induced GATA-1 expression in U937-NDRG2 cells. The results reveal that NDRG2 promotes the expression of GATA-1 through activation of the JAK2/STAT pathway, but not through the regulation of the BMP-4/Smad pathway in U937 cells. Our findings further suggest that NDRG2 may play a role as a regulator of erythrocyte and megaka...Continue Reading

References

Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato
Sep 5, 2001·Current Opinion in Genetics & Development·A B Cantor, S H Orkin
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Gary Thomas
Jul 2, 2003·Molecular and Cellular Biology·Marcin RylskiMitchell J Weiss
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Yanchun DengMarie Chia-mi Lin
Jun 23, 2004·Neurobiology of Disease·Cathy MitchelmoreNiels A Jensen
Sep 29, 2004·Molecular and Cellular Endocrinology·Craig A HarrisonWylie Vale
Jul 26, 2005·Neuroscience Letters·Kou TakahashiMitsuhiko Yamada
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Xin-Hua Feng, Rik Derynck
Jun 5, 2007·Blood·Alvin C H MaAnskar Y H Leung
Sep 25, 2007·Biochemical and Biophysical Research Communications·Yongjin ParkJong-Seok Lim
Oct 16, 2007·Oncogene·A Wickrema, J D Crispino
Dec 18, 2007·Nature Chemical Biology·Gregory J Anderson, Deepak Darshan
May 20, 2009·Biochemical and Biophysical Research Communications·Soo-Kyung ShonJong-Seok Lim
Jun 27, 2009·The Journal of Biological Chemistry·Chun FanQing Kenneth Wang
Aug 5, 2009·The Journal of Biological Chemistry·Sylvia M Nelsen, Jan L Christian
Sep 24, 2009·Experimental Hematology·Alvin C H MaAnskar Y H Leung
Apr 23, 2010·Blood·Aurelie de ThonelCarmen Garrido
May 5, 2010·Biochemical and Biophysical Research Communications·Seung-Chul ChoiJae Wha Kim
Jul 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Veerle MelotteManon van Engeland
Jul 31, 2010·The Journal of Biological Chemistry·Emery H BresnickSunduz Keles
Oct 19, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Donghua HeZhen Cai
Feb 3, 2011·Immune Network·Eun Byul LeeJong-Seok Lim
Mar 11, 2011·Annals of the New York Academy of Sciences·Nabil G Seidah

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Citations

Dec 14, 2019·Cellular and Molecular Life Sciences : CMLS·Xin LiLize Xiong
Feb 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Zhi-Guo ZhangLin Wu

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Methods Mentioned

BETA
ubiquitination
PMA
transfection
PCR

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