DOI: 10.1101/473397Nov 22, 2018Paper

NDRG2 Regulates Adherens Junction Integrity to Restrict Colitis and Tumorigenesis

BioRxiv : the Preprint Server for Biology
Yongzheng Majian zhang


Objective: Paracellular barriers play an important role in the pathogenesis of IBDs, such as ulcerative colitis (UC) and Crohns disease (CD), and maintain gut homeostasis. N-myc downstream-regulated gene 2 (NDRG2) has been reported to be a tumor suppressor gene and inhibits epithelial-mesenchymal transition (EMT) and colorectal cancer metastasis. However, whether NDRG2 affects colitis initiation and colitis-associated colorectal cancer is unclear. Our study aimed to elucidate the role of NDRG2 in colitis initiation and colitis-associated colorectal cancer using an intestine-specific Ndrg2 knockout mouse model. Design: We generated intestine-specific Ndrg2 knockout mice and assessed the function of Ndrg2 in established experimental colitis and colitis-associated tumor models. Results: Intestine-specific Ndrg2 deficiency caused mild spontaneous colitis with ageing, more severe inflammation in dextran sodium sulfate (DSS) and 2,4,6-Trinitrobenzenesulfonic acid (TNBS) treated mice and enhanced pro-inflammatory cytokine levels, neutrophil and macrophage recruitment. Ndrg2 loss led to adherens junction (AJ) structure destruction via E-cadherin expression attenuation, resulting in diminished epithelial barrier function and increased i...Continue Reading

Related Concepts

Inflammatory Bowel Diseases
Ubiquitin-protein ligase
Tumor Suppressor Genes
N-Myc Protein
Mice, Knockout

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.