NDRG4, an early detection marker for colorectal cancer, is specifically expressed in enteric neurons

Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
N VaesVeerle Melotte

Abstract

Promoter methylation of N-myc Downstream-Regulated Gene 4 (NDRG4) in fecal DNA is an established early detection marker for colorectal cancer (CRC). Despite its connection to CRC, NDRG4 is predominantly studied in brain and heart, with little to no knowledge about its expression or role in other organs. In this study, we aimed to determine the whole-body expression of NDRG4, with a focus on the intestinal tract. We investigated NDRG4 expression throughout the body by immunohistochemistry, Western Blotting and in situ mRNA hybridization using tissues from NDRG4 wild-type, heterozygous and knockout mice and humans. In addition, we explored cell-specific expression of NDRG4 in murine whole-mount gut preparations using immunofluorescence and confocal microscopy. NDRG4 is specifically expressed within nervous system structures throughout the body. In the intestinal tract of both mouse and man, NDRG4 immunoreactivity was restricted to the enteric nervous system (ENS), where it labeled cell bodies of the myenteric and submucosal plexuses and interconnecting nerve fibers. More precisely, NDRG4 expression was limited to neurons, as NDRG4 always co-localized with HuC/D (pan-neuronal marker) but never with GFAP (an enteric glial cell mark...Continue Reading

References

Apr 30, 2002·Brain Research. Developmental Brain Research·Noriyuki NakadaMinoru Takeda
Apr 30, 2002·Brain Research. Developmental Brain Research·Takeshi OhkiMinoru Takeda
Apr 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tiffany A Heanue, Vassilis Pachnis
Jul 28, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lawrence C R TafoyaMichael C Wilson
Nov 14, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tomohiko OkudaToshiyuki Miyata
May 22, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·V VillanacciK Geboes
Sep 18, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wisuit PradidarcheepWouter H Lamers
Mar 24, 2009·Nature Genetics·Christopher Newton-ChehBruno H Ch Stricker
Jun 19, 2009·Journal of the National Cancer Institute·Veerle MelotteManon van Engeland
May 5, 2010·Biochemical and Biophysical Research Communications·Seung-Chul ChoiJae Wha Kim
Jul 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Veerle MelotteManon van Engeland
Mar 24, 2011·Journal of Cellular and Molecular Medicine·Nunzia BernardiniAmelio Dolfi
Jun 4, 2011·Gastroenterology·Kara Gross MargolisMichael David Gershon
Jun 4, 2011·The Journal of Biological Chemistry·Hitomi YamamotoToshiyuki Miyata
Nov 9, 2011·Gastroenterology·David A AhlquistGraham P Lidgard
Mar 7, 2012·Nature Reviews. Gastroenterology & Hepatology·John B Furness
Nov 21, 2012·Nature Reviews. Gastroenterology & Hepatology·Michel NeunlistMalvyne Rolli-Derkinderen
Jul 13, 2013·Science·Claire MagnonPaul S Frenette
Mar 22, 2014·The New England Journal of Medicine·Thomas F ImperialeBarry M Berger
Jun 19, 2014·Molecular Neurodegeneration·Sarah HerdewynPhilip Van Damme
Jan 24, 2016·Biochemical and Biophysical Research Communications·Xianghu QuH Scott Baldwin
Dec 3, 2016·PLoS Genetics·Laura FontenasMarcel Tawk

❮ Previous
Next ❯

Citations

Oct 28, 2019·Reviews in Endocrine & Metabolic Disorders·Estelle Grasset, Remy Burcelin
Oct 2, 2021·Oncology Letters·Ruikai LiJianyong Zheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Neuroscience Research
Mia M ThiMenachem Hanani
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Guy R SanderBarry C Powell
© 2022 Meta ULC. All rights reserved