Near infrared fluorescence imaging of EGFR expression in vivo using IRDye800CW-nimotuzumab

Oncotarget
Wendy BernhardC Ronald Geyer

Abstract

Nimotuzumab is a humanized anti-epidermal growth factor receptor (EGFR) monoclonal antibody that is approved in many countries for the treatment of EGFR-positive cancers. Near infrared (NIR) fluorescent dye-labeled antibodies represent an attractive class of image-guided surgical probes because of their high specificity, tumor uptake, and low dissociation from tumor cells that express the antigen. In this study, we developed a NIR fluorescent dye-labeled nimotuzumab immunoconjugate, IRDye800CW-nimotuzumab, and evaluated in vitro binding with EGFR-positive cells, in vivo tumor uptake by NIR fluorescent imaging, and ex vivo biodistribution. There was no difference in binding between nimotuzumab and IRDye800CW-nimotuzumab to EGFR-positive cells. In mice bearing EGFR-positive xenografts, IRDye800CW-nimotuzumab uptake peaked at 4 days post injection and slowly decreased thereafter with high levels of accumulation still observed at 28 days post injection. In EGFR-positive xenografts, IRDye800CW-nimotuzumab showed more than 2-fold higher uptake in tumors compared to IRDye800CW-cetuximab. In addition, liver uptake of IRDye800CW-nimotuzumab was two-fold lower than cetuximab. The lower liver uptake of IRDye800CW-nimotuzumab could have im...Continue Reading

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Citations

Jun 28, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Md Kausar AlamC Ronald Geyer
Apr 22, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Prashant DograZhihui Wang
Sep 5, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rahul PalAnand T N Kumar

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Methods Mentioned

BETA
surgical resection
flow cytometry
xenografts
xenograft
electrophoresis
dissection
fluorescence imaging

Software Mentioned

Image Studio
Flowjo
Prism6
Data Analysis

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