Near-Infrared Fluorescent Turn-on Probe with a Remarkable Large Stokes Shift for Imaging Selenocysteine in Living Cells and Animals

Analytical Chemistry
Weiyong FengGuoqiang Feng

Abstract

Selenocysteine (Sec) is the 21st naturally occurring amino acid and has emerged as an important sensing target in recent years. However, fluorescent detection of Sec in living systems is challenging. To date, very few fluorescent Sec probes have been reported and most of them respond fluorescence to Sec in the visible region. In this paper, a very promising near-infrared fluorescent probe for Sec was developed. This probe works in aqueous solution over a wide pH range under mild conditions and can be used for rapid, highly selective and sensitive detection of Sec with significant near-infrared fluorescent turn-on signal changes. In addition, it features a remarkable large Stokes shift (192 nm) and a low detection limit (60 nM) for Sec with a wide linear range (0-70 μM). Moreover, this probe can be conveniently used to detect Sec in serum samples, living cells, and animals, indicating it holds great promise for biological applications.

References

Mar 1, 1991·Molecular Microbiology·A BöckF Zinoni
Oct 31, 2001·Public Health Nutrition·K M Brown, J R Arthur
May 31, 2003·Science·Gregory V KryukovVadim N Gladyshev
May 19, 2007·Antioxidants & Redox Signaling·Laura Vanda PappKum Kum Khanna
Sep 4, 2010·Antioxidants & Redox Signaling·Susan J Fairweather-TaitRachel Hurst
Mar 3, 2012·Lancet·Margaret P Rayman
Apr 4, 2013·Chemical Communications : Chem Comm·Wen SunXiaojun Peng
Feb 4, 2014·Trends in Biochemical Sciences·Dolph L HatfieldVadim N Gladyshev
Feb 15, 2014·Journal of the American Chemical Society·Xumeng WuWeihong Zhu
Jul 6, 2014·Physiological Reviews·Vyacheslav M LabunskyyVadim N Gladyshev
Jul 12, 2014·Chemical Communications : Chem Comm·Jiangli FanJianfang Cao
Jan 7, 2015·Journal of the American Chemical Society·Baoxin ZhangJianguo Fang
Jan 20, 2015·Chemical Communications : Chem Comm·Fanpeng KongBo Tang
Mar 8, 2016·ACS Chemical Biology·Hans J Reich, Robert J Hondal

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Citations

Sep 12, 2018·Chemistry, an Asian Journal·Baoxin ZhangJianguo Fang
Nov 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Weijie ZhangCaixia Yin
May 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dayoung LeeJuyoung Yoon
Nov 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Meng-Zhao ZhangWei-Hong Zhu
Nov 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zongcheng WangYuren Jiang

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