Near-Infrared Light-Initiated Hybridization Chain Reaction for Spatially and Temporally Resolved Signal Amplification

Angewandte Chemie
Hongqian ChuLele Li

Abstract

Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled amplification has not been realized because of the lack of a design methodology. The aim of this study was thus to develop a conceptual approach for remote control over signal amplification at a chosen time and site in living cells. This system was constructed by re-engineering the functional units of the hybridization chain reaction (HCR) and combination with upconversion photochemistry, thus resulting in an activatable HCR with the high spatial and temporal precision of near-infrared (NIR) light. As a proof of concept, we demonstrate the spatially and temporally resolved amplified imaging of messenger RNA (mRNA) with ultrahigh sensitivity in vitro and in vivo. Furthermore, by using a system targeting subcellular sites we have developed a new technique for NIR-initiated amplified imaging of mRNA exclusively within a specific organelle.

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Citations

Jun 19, 2021·ACS Synthetic Biology·Cole EmanuelsonAlexander Deiters
Jun 27, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yue ZhangHuangxian Ju
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia
Jul 21, 2021·Biosensors & Bioelectronics·Jiani DongQubo Zhu
Jul 30, 2021·Journal of the American Chemical Society·Zhian HuXinrong Zhang
Aug 11, 2021·ACS Applied Materials & Interfaces·Hua ChaiPeng Miao
Aug 9, 2021·Talanta·Jingting WuZai-Sheng Wu
Dec 5, 2020·Analytical Chemistry·Xueli ZhuJian-Hui Jiang
Feb 27, 2020·ACS Applied Materials & Interfaces·Mei-Rong CuiHong-Yuan Chen
Jan 23, 2020·Journal of the American Chemical Society·Kewei RenMingxu You
Feb 19, 2020·Analytical Chemistry·Mei-Rong CuiHong-Yuan Chen
Sep 14, 2021·Journal of the American Chemical Society·Yue ZhangHuangxian Ju

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