Near-infrared photoimmunotherapy targeting EGFR-Shedding new light on glioblastoma treatment

International Journal of Cancer. Journal International Du Cancer
Thomas A BurleyGabriela Kramer-Marek

Abstract

Glioblastomas (GBMs) are high-grade brain tumors, differentially driven by alterations (amplification, deletion or missense mutations) in the epidermal growth factor receptor (EGFR), that carry a poor prognosis of just 12-15 months following standard therapy. A combination of interventions targeting tumor-specific cell surface regulators along with convergent downstream signaling pathways may enhance treatment efficacy. Against this background, we investigated a novel photoimmunotherapy approach combining the cytotoxicity of photodynamic therapy with the specificity of immunotherapy. An EGFR-specific affibody (ZEGFR:03115 ) was conjugated to the phthalocyanine dye, IR700DX, which when excited with near-infrared light produces a cytotoxic response. ZEGFR:03115 -IR700DX EGFR-specific binding was confirmed by flow cytometry and confocal microscopy. The conjugate showed effective targeting of EGFR positive GBM cells in the brain. The therapeutic potential of the conjugate was assessed both in vitro, in GBM cell lines and spheroids by the CellTiter-Glo® assay, and in vivo using subcutaneous U87-MGvIII xenografts. In addition, mice were imaged pre- and post-PIT using the IVIS/Spectrum/CT to monitor treatment response. Binding of the ...Continue Reading

References

Jul 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Paul H HuangForest M White
Oct 14, 2008·Journal of Neurosurgery·Matthew J McGirtAlf Redo Quiñones-Hinojosa
Jun 16, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Gabriela Kramer-MarekJacek Capala
May 27, 2010·British Journal of Cancer·P WorkmanUNKNOWN Committee of the National Cancer Research Institute
Jun 1, 2010·Photodiagnosis and Photodynamic Therapy·Ron R Allison, Claudio H Sibata
Aug 4, 2012·Cancer Treatment Reviews·Denise BechetMuriel A Barberi-Heyob
Jan 25, 2014·Nature Reviews. Cancer·Dominik SturmStefan M Pfister
Apr 10, 2015·Nature Reviews. Cancer·Frank B FurnariPaul S Mischel
May 30, 2015·Cancer Letters·Kazuhide SatoHisataka Kobayashi
Jun 16, 2015·British Journal of Neurosurgery·Jason M WarramEben L Rosenthal
Jun 9, 2016·Nature Genetics·Jiguang WangRaul Rabadan
Jul 6, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ana Luiza Ribeiro de SouzaBrian W Pogue
Nov 2, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jonathan T ElliottBrian W Pogue
Jan 18, 2017·Nature Reviews. Clinical Oncology·Ray R ZhangJamey P Weichert
Mar 21, 2017·BioMed Research International·Wojciech SzopaWojciech Kaspera

❮ Previous
Next ❯

Citations

Jan 17, 2019·Organic & Biomolecular Chemistry·Sara R G FernandesJoão P C Tomé
Nov 24, 2019·International Journal of Molecular Sciences·Haruka YamaguchiAndreas Evdokiou
Jan 10, 2019·World Journal of Gastroenterology : WJG·Winn AungTatsuya Higashi
Jan 24, 2019·International Journal of Molecular Sciences·Makoto TsuchimochiHirofumi Fujii
May 30, 2020·Acta Pharmacologica Sinica·Sumaira HanifBing-Yang Shi
Nov 19, 2019·Frontiers in Chemistry·Claudia FerroniGreta Varchi
Sep 10, 2020·Biomedicines·Fleury Augustin Nsole BitegheStefan Barth
Oct 22, 2020·Cell Death & Disease·Justyna MączyńskaGabriela Kramer-Marek
Mar 21, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bernhard KissJoseph C Liao
Mar 29, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Deepak S ChauhanMurali M Yallapu
Aug 7, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chengqiong MaoXin Ming
Dec 24, 2020·Expert Opinion on Biological Therapy·Micol Silic-BenussiMauro Alaibac
Jun 3, 2021·International Journal of Pharmaceutics·Fang LiXin Ming
Jun 3, 2021·Cancers·Takuya KatoHisataka Kobayashi
Feb 16, 2019·Bioconjugate Chemistry·Jordon Sandland, Ross W Boyle
Dec 4, 2021·Expert Opinion on Biological Therapy·Irene RussoMauro Alaibac
Jul 30, 2020·Anti-cancer Agents in Medicinal Chemistry·Suresh MadheswaranStefan Barth

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Confocal microscopy
xenografts
Fluorescence imaging
Fluorescence
xenograft

Clinical Trials Mentioned

NCT02422979

Software Mentioned

IVIS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.