Near-infrared reflectance analysis to evaluate the nitrogen and fat content of human milk in neonatal intensive care units

Archives of Disease in Childhood. Fetal and Neonatal Edition
L CorvagliaGiacomo Faldella

Abstract

To validate near-infrared reflectance analysis (NIRA) as a fast, reliable and suitable method for routine evaluation of human milk's nitrogen and fat content. One neonatal intensive care unit. 124 samples of expressed human milk (55 from preterm mothers and 69 from term mothers). Measurement of nitrogen and fat content by NIRA and traditional methods (Gerber method for fat and Kjeldahl method for nitrogen). Agreement between NIRA and traditional methods. Variability in fat and nitrogen content of human milk. A strong agreement was found between the results of traditional methods and NIRA for both fat and nitrogen content (expressed as g/100 g of milk) in term (mean fat content: NIRA = 2.76; Gerber = 2.76; mean nitrogen content: NIRA = 1.88; Kjeldahl = 1.92) and preterm (mean fat content: NIRA = 3.56; Gerber = 3.52; mean nitrogen content: NIRA = 1.91; Kjeldahl = 1.89) mothers' milk. Nitrogen content of the milk samples, measured by NIRA, ranged from 1.18 g/100 g to 2.71 g/100 g of milk in preterm milk and from 1.48 g/100 g to 2.47 g/100 g in term milk; fat content ranged from 1.27 g/100 g to 6.23 g/100 g of milk in preterm milk and from 1.01 g/100 g to 6.01 g/100 g of milk in term milk. NIRA can be used as a quick and reliable t...Continue Reading

Citations

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