Near-infrared spectroscopy for metabolite quantification and species identification

Ecology and Evolution
Wen C Aw, J William O Ballard

Abstract

Near-infrared (NIR) spectroscopy is a high-throughput method to analyze the near-infrared region of the electromagnetic spectrum. It detects the absorption of light by molecular bonds and can be used with live insects. In this study, we investigate the accuracy of NIR spectroscopy in determining triglyceride level and species of wild-caught Drosophila. We employ the chemometric approach to produce a multivariate calibration model. The multivariate calibration model is the mathematical relationship between the changes in NIR spectra and the property of interest as determined by the reference analytical method. Once the calibration model was developed, we used an independent set to validate the accuracy of the calibration model. The optimized calibration model for triglyceride quantification yielded coefficients of determination of 0.73 for the calibration test set and 0.70 for the independent test set. Simultaneously, we used NIR spectroscopy to discriminate two species of Drosophila. Flies from independent sets were correctly classified into Drosophila melanogaster and Drosophila simulans with accuracy higher than 80%. These results suggest that NIRS has the potential to be used as a high-throughput screening method to assess a...Continue Reading

Associated Datasets

References

Apr 21, 2001·Journal of Agricultural and Food Chemistry·F B Ahmad, P A Williams
May 23, 2001·Journal of Agricultural and Food Chemistry·J B Reeves
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Dec 17, 2003·Journal of Gastroenterology and Hepatology·Kevin DolanRobert Sutton
Oct 10, 2009·The American Journal of Tropical Medicine and Hygiene·Valeliana S MayagayaFloyd E Dowell
Apr 10, 2012·Journal of Pharmaceutical and Biomedical Analysis·Ana Carolina de Oliveira NevesKássio Michell Gomes de Lima
Aug 4, 2012·Journal of Biosocial Science·Boaventura M CauVictor Agadjanian
Aug 24, 2012·Biology Letters·H Colinet, D Renault
Dec 25, 2012·Developmental Cell·Xue Li GuanMarkus R Wenk
Jul 3, 2013·Contemporary Clinical Trials·Jyothi Subramanian, Richard Simon
Mar 19, 2014·Methods : a Companion to Methods in Enzymology·Jason M TennessenCarl S Thummel
Jun 3, 2014·BMC Evolutionary Biology·Hannah M E TennantTristan A f Long
Mar 25, 2015·Journal of Biomedical Optics·Robert H WilsonAnthony J Durkin
Mar 18, 2016·Annual Review of Entomology·Christian Nansen, Norman Elliott
Oct 22, 2016·International Journal of Legal Medicine·Sasha C VossChristian Nansen

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GRAMS IQ Predict
Asd to Spc convertor
RS
GRAMS IQ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.