Near-UV circular dichroism of trypsin inhibitor of adzuki beans attributable to disulfide groups

Journal of Biochemistry
C YoshidaT Takagi

Abstract

The trypsin inhibitor of adzuki (Phaseolus angularis) beans shows a CD spectrum with a negative extremum at 280 nm and a positive shoulder around 245 nm. Since the inhibitor lacks tryptophan and tyrosine, the observed CD spectrum can be attributed to the six disulfide groups in the molecule. The CD features completely disappeared on reduction of the disulfide groups, and converged into a single negative extremum at 270 nm when the groups were modified to form mixed disulfides with glutathione. These observations of the CD properties of the inhibitor strongly suggest the presence of disulfide groups constrained with respect to their dihedral angles.

Citations

Nov 1, 1978·International Journal of Peptide and Protein Research·T A Bewley, Y Birk
Mar 1, 1982·International Journal of Peptide and Protein Research·M SogamiH Inouye
Mar 1, 1980·International Journal of Peptide and Protein Research·Y BirkT A Bewley
Apr 1, 1983·International Journal of Peptide and Protein Research·T W Strickland, D Puett

Related Concepts

Circular Dichroism, Vibrational
Disulfides
Reduced Glutathione
Hydrogen-Ion Concentration
Trypsin Inhibitors

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