Nebrodeolysin, a novel hemolytic protein from mushroom Pleurotus nebrodensis with apoptosis-inducing and anti-HIV-1 effects

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Hui LvJin-ku Bao

Abstract

A novel hemolysin was isolated from the edible mushroom Pleurotus nebrodensis by ion exchange and gel filtration chromatography on DEAE-Sepharose and Sephacryl S-100. The hemolysin from Pleurotus nebrodensis hemolysin (nebrodeolysin) is a monomeric protein with a molecular weight of approximately 27 kDa as determined by gel filtration and SDS-PAGE. Nebrodeolysin exhibited remarkable hemolytic activity towards rabbit erythrocytes and caused efflux of potassium ions from erythrocytes. Subsequently, this hemolysin showed strong cytotoxicity against Lu-04, Bre-04, HepG2, L929, and HeLa cells. It was also found that this hemolysin induced apoptosis in L929 and HeLa cells as evidenced by microscopic observations and DNA ladder, respectively. Moreover, this hemolysin was shown to possess anti-HIV-1 activity in CEM cell culture.

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Citations

Jul 10, 2012·Medical Mycology·Ajay P NayakDonald H Beezhold
Mar 22, 2014·Journal of Ethnopharmacology·Alfred Mugambi MarigaQiu-hui Hu
Jun 25, 2013·Expert Opinion on Investigational Drugs·Su-Juan WangLu-Ping Qin
Jul 17, 2015·BioMed Research International·Fabíola Dorneles InácioCristina Giatti Marques de Souza
Dec 29, 2011·Fungal Genetics and Biology : FG & B·Weiwei KongJinxia Zhang
Jul 14, 2010·FEMS Microbiology Letters·Chun-Hua HanTzi Bun Ng
Sep 20, 2019·BMC Complementary and Alternative Medicine·Kavithambigai EllanVikineswary Sabaratnam
Jun 20, 2020·Applied Microbiology and Biotechnology·Vala RezvaniLeila Farahmand
Sep 21, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sze Kwan Lam, Tzi Bun Ng
Dec 11, 2021·Journal of Food Biochemistry·Jackson IsharaJohn Kinyuru

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