Nebulette: a 107 kD nebulin-like protein in cardiac muscle

Cell Motility and the Cytoskeleton
C L Moncman, K Wang

Abstract

A 107-kD protein has been identified in primary cultures of chicken embryonic cardiomyocytes by immunoprecipitations with certain anti-nebulin monoclonal antibodies (mAbs). These mAbs, prepared against a fragment of human skeletal muscle nebulin located near the carboxyl terminus, detect a 107-kD protein in extracts of adult chicken heart, adult mouse heart, and adult rabbit heart by immunoblot analysis. A partial cDNA corresponding to this protein has been isolated by immunological screening of a chicken heart cDNA expression vector library. The partial cDNA encodes a 380-amino acid open reading frame composed entirely of nebulin-like 35-residue modules marked by the highly conserved sequence motifs: SXXXYK and TPD. The open reading frame exhibits 60-85% homology with skeletal muscle nebulins from a variety of species. This cDNA recognizes an approximately 8-kb transcript in cardiac RNA and does not hybridize to skeletal muscle RNAs by northern analysis. Immunofluorescence localization of this nebulin-like protein in primary cultures of chicken cardiomyocytes and embryonic chicken cardiac myofibrils indicates that the protein is localized to the I-Z-I complex of the myofibrils, extending approximately 25% of the thin filament ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K WangA Tu
Jan 1, 1979·The Journal of Physiology·T F Robinson, S Winegrad
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 1, 1991·The Journal of Cell Biology·T J ByersS C Watkins
May 6, 1991·FEBS Letters·S LabeitJ Trinick
Aug 1, 1991·Experimental Cell Research·K J Doane, D E Birk
Feb 1, 1986·Analytical Biochemistry·R M Amasino
Dec 1, 1989·Journal of Muscle Research and Cell Motility·S Pierobon-BormioliG Salviati
Jan 1, 1989·Methods in Enzymology·K WangJ V Staros
Jul 1, 1987·Archives of Biochemistry and Biophysics·S M MorrisW E O'Brien
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·C C LiewC Y Lee
Jan 1, 1994·Cell Motility and the Cytoskeleton·D RheeJ W Sanger
Feb 1, 1994·Current Opinion in Cell Biology·K R Fath, D R Burgess
Oct 1, 1993·Molecular Biology of the Cell·C L MoncmanD A Winkelmann

❮ Previous
Next ❯

Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·K K TurnaciogluJ W Sanger
Jan 1, 1996·Cell Motility and the Cytoskeleton·C L Moncman, K Wang
Mar 8, 2000·Cell Motility and the Cytoskeleton·A H HerreraR Horowits
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·M GautelP Young
Jan 18, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Derk FrankNorbert Frey
Oct 21, 2006·Cell and Tissue Research·Carole L Moncman, Francisco H Andrade
May 1, 1996·Trends in Cardiovascular Medicine·C C Gregorio, V M Fowler
May 6, 2003·Journal of Molecular Biology·Steven T KazmierskiCarol C Gregorio
Aug 26, 2000·Progress in Biophysics and Molecular Biology·R Bottinelli, C Reggiani
Feb 1, 1996·Current Opinion in Cell Biology·V M Fowler
Feb 27, 1999·Current Opinion in Cell Biology·C C GregorioS Labeit
Apr 1, 1997·Current Opinion in Structural Biology·J M Squire
Jul 3, 2003·Trends in Cardiovascular Medicine·Abigail S McElhinnyCarol C Gregorio
Jan 10, 2013·Nature Reviews. Molecular Cell Biology·David S Gokhin, Velia M Fowler
Feb 8, 2013·Molecular Biology of the Cell·Katherine T BlissCarol C Gregorio
Nov 17, 2007·Cardiovascular Research·Samuel Y Boateng, Paul H Goldspink
Dec 1, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Siegfried LabeitHenk Granzier
Jan 19, 1999·Annual Review of Cell and Developmental Biology·R Littlefield, V M Fowler
May 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Catherine S ChewHan Zhang
Nov 26, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Laura A A GilliamMichael B Reid
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·K PelinC Wallgren-Pettersson
Nov 9, 2005·Cytogenetic and Genome Research·A G TerasakiK Ohashi
Sep 15, 2005·The Journal of Cell Biology·Abigail S McElhinnyCarol C Gregorio
Apr 7, 2010·The Journal of Cell Biology·David S GokhinVelia M Fowler
Jul 13, 2001·The Journal of Cell Biology·J W Sanger, J M Sanger
Oct 19, 2010·Trends in Cell Biology·Christopher T PappasCarol C Gregorio
Mar 31, 2010·Experimental Cell Research·Carole L Moncman, Francisco H Andrade
Sep 1, 2015·Circulation Journal : Official Journal of the Japanese Circulation Society·Marie-Louise Bang, Ju Chen
Apr 6, 2005·Cell Motility and the Cytoskeleton·Jushuo WangJoseph W Sanger
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elisabeth EhlerJean-Claude Perriard
Nov 8, 2007·Cell Motility and the Cytoskeleton·William B Holmes, Carole L Moncman
Jan 24, 2007·Cell Motility and the Cytoskeleton·Michael EshamCarole L Moncman
Feb 28, 2008·Cell Motility and the Cytoskeleton·Jushuo WangJoseph W Sanger
Aug 5, 2008·Cell Motility and the Cytoskeleton·Xiaodi A DengCarole L Moncman
Dec 22, 2006·Cell Motility and the Cytoskeleton·Melissa A SenetarRichard O McCann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.