Necroptosis contributes to the NMDA-induced excitotoxicity in rat's cultured cortical neurons

Neuroscience Letters
Yanli LiCe Zhang

Abstract

Necroptosis is a newly discovered type of cell death characterized with the combined biochemical and ultrastructural features of necrosis and apoptosis. Nec-1 has been reported to exhibit the selective inhibition for necroptosis, which has now been used as an operational definition of necroptosis. The purpose of this study was to evaluate whether necroptosis is involved in the NMDA-induced excitotoxicity and furthermore whether the elevation of intracellular Ca(2+) is involved in the NMDA-induced necroptosis. Our findings showed that Nec-1 (100 micromol/L) inhibited NMDA-induced decrease of cell viability by 26% (p<0.01), suppressed NMDA-induced decrease of living cells by 23% (p<0.01) and attenuated NMDA-induced leakage of LDH by 28% (p<0.01). In addition, Nec-1 also suppressed NMDA-induced elevation of intracellular Ca(2+) by 36% (p<0.05). These findings indicated for the first time that necroptosis contributes to the NMDA-induced excitotoxicity, and the elevation of intracellular Ca(2+) may be one of the potential mechanisms underlying NMDA-induced necroptosis. Although necroptosis may be involved in NMDA-induced excitotoxicity, it may just constitute a small contribution.

References

Oct 1, 1992·Current Opinion in Neurobiology·M F Beal
Mar 20, 2001·Archives of Neurology·N J Maragakis, J D Rothstein
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Sten OrreniusPierluigi Nicotera
Mar 24, 2005·Infection and Immunity·Susan L Fink, Brad T Cookson

❮ Previous
Next ❯

Citations

Jul 21, 2011·Pathology Oncology Research : POR·Zsuzsanna DunaiRudolf Mihalik
Nov 15, 2011·Cell Death and Differentiation·N VanlangenakkerP Vandenabeele
Feb 24, 2012·Human Molecular Genetics·Ishaq A ViringipurampeerCheryl Y Gregory-Evans
Dec 3, 2010·Genes & Development·Junying Yuan, Guido Kroemer
Jul 28, 2012·International Journal of Molecular Sciences·Weidong HanHongming Pan
Mar 1, 2014·Cell Death & Disease·Q LiuM J Whalen
May 14, 2011·Journal of Cellular and Molecular Medicine·Christopher C T Smith, Derek M Yellon
Oct 23, 2010·Journal of Internal Medicine·B Zhivotovsky, S Orrenius
Nov 20, 2015·Cell Death & Disease·H ZhaoD Ma
Jan 28, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Yuan-Yuan Zhang, Hao Liu
Jan 21, 2012·Renal Failure·Vivian Regina TristãoJúlio Cesar Martins Monte
Oct 4, 2011·Critical Reviews in Oncology/hematology·Wei WuJianyong Li
Oct 21, 2016·Expert Opinion on Therapeutic Patents·Spandana Rajendra KopalliSushruta Koppula
Jul 31, 2012·Acta Biochimica Et Biophysica Sinica·Hongling HuangXin-Yuan Liu
Jun 12, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kun XiongJie Yan
Feb 26, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T LiuJ Y Jiang
Sep 16, 2009·Biochimica Et Biophysica Acta·Phillip NagleyPhilip M Beart
Jan 14, 2017·Neurochemistry International·Stephanie J GuineyScott Ayton
Oct 3, 2018·International Journal of Molecular Sciences·Xiaorong YangCe Zhang
Oct 20, 2018·Journal of Cell Science·Diego E HernándezFelipe A Court
Aug 28, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lixuan ZhanEn Xu
Nov 1, 2015·Apoptosis : an International Journal on Programmed Cell Death·Vivian Regina TristãoJúlio Cesar Martins Monte
Sep 26, 2012·British Journal of Pharmacology·Gang ChengJian-ning Zhang
Oct 10, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A LinkermannA M Jevnikar
Jul 8, 2015·Expert Review of Hematology·Yongfeng ChenYong Liang
Apr 10, 2014·Archives of Pharmacal Research·Ji Hyun BaeYoung Sik Cho
Mar 29, 2014·International Journal of Molecular Medicine·Young Sik Cho
Mar 26, 2011·IUBMB Life·Laure DelavalléeSantos A Susin
Jul 14, 2012·Journal of Oral Science·Hiromasa TsudaNaoto Suzuki
Oct 31, 2017·Oxidative Medicine and Cellular Longevity·Xiaorong YangCe Zhang
Jul 4, 2018·Signal Transduction and Targeted Therapy·Qi ChenCaiyun Fu
Mar 1, 2018·Physiological Reviews·Michael FrickerGuy C Brown
Apr 10, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Linsen Xie, Yongjie Huang
Jan 12, 2021·Frontiers in Cellular Neuroscience·Shuheng WenKoichi Uemura
Apr 1, 2010·Science Signaling·Peter VandenabeeleTom Vanden Berghe
Oct 16, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Tatsuki KoikeTakanobu Kuroita

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis