Necrosis-like death with plasma membrane damage against cervical cancer cells by photodynamic therapy

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
W S AhnY-W Kim

Abstract

In order to elucidate the antitumor effect of photodynamic therapy (PDT) using the photosensitizing agent hematoporphyrin derivative (Photogem) and a diode laser, we evaluated the cell death of uterine cancer cell lines (CaSki, HT3, HeLa, and SKOV-3) and mice transplanted with TC-1 lung cancer cells. Morphological changes, MTT assay, flow cytometry, cytotoxicity, and tumor growth-inhibition study were evaluated at various time intervals after PDT. The results showed that the survival rates of each cell line decreased with time and dose-response after performing PDT. Also, PDT-induced damage of cancer cells was almost entirely confined to necrosis of the tumor cells in the early time courses. The irradiation of CaSki cells in the presence of Photogem induced plasma membrane disruption and cell shrinkage, indicating the plasma membrane as the main target for Photogem. In the experiment in vivo, the time courses of Photogem with irradiation showed significantly longer survival and a significantly smaller tumor size compared to those in the untreated control groups, and resorption of the tumor after PDT treatment was observed. Collectively, our results indicated that Photogem possesses tumor-specific affinity, and necrosis-like dea...Continue Reading

References

Jun 30, 1992·Biochimica Et Biophysica Acta·L C PenningJ Van Steveninck
Jan 1, 1992·Photochemistry and Photobiology·B W Henderson, T J Dougherty
Apr 1, 1994·Photochemistry and Photobiology·X Y HeS L Jacques
Nov 1, 1996·Photochemistry and Photobiology·J G Levy, M Obochi
Jun 24, 1998·Journal of the National Cancer Institute·T J DoughertyQ Peng
Mar 19, 1999·Cytometry·E BednerZ Darzynkiewicz
May 10, 2000·Nature Medicine·K Birmingham, M Watanabe
May 10, 2000·Nature Medicine·B Renault
Dec 2, 2000·Journal of Photochemistry and Photobiology. B, Biology·A C Moor
Apr 21, 2001·Cellular and Molecular Life Sciences : CMLS·G DeneckerP Vandenabeele
Mar 21, 2002·Journal of Clinical Laser Medicine & Surgery·Thomas J Dougherty
Apr 27, 2002·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Hiroshi NakamuraK Aizawa

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Citations

Feb 18, 2009·The Journal of Biological Chemistry·Nataly Kravchenko-BalashaAlexander Levitzki
Jun 8, 2006·Archives of Pharmacal Research·Sang Jin JiJeong Hee Kim
Sep 8, 2007·The International Journal of Biochemistry & Cell Biology·Louise CopleyVirna D Leaner
Sep 26, 2012·Cell Biology International·Krishna V Sharma, Lester M Davids
Dec 2, 2009·Cell Biology International·Ana Paula Dias RibeiroCarlos Alberto de Souza Costa
Sep 23, 2009·IEEE Transactions on Bio-medical Engineering·Arisa ItoTsunenori Arai
Mar 19, 2014·Photodiagnosis and Photodynamic Therapy·Seong Taek MunWoong Shick Ahn
Jan 25, 2011·Photomedicine and Laser Surgery·Luis Fernando TirapelliCarlos Gilberto Carlotti Junior
Nov 22, 2008·Journal of Biomedical Optics·Nagapratima KunapareddyJudith R Mourant
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Oyunbileg GalindevYoung Key Shim
Jun 21, 2005·Lasers in Surgery and Medicine·Ahmad KavianiGholamreza Esmaeeli Djavid
Nov 15, 2007·Virchows Archiv : an International Journal of Pathology·Enrique LermaJaime Prat
Jul 16, 2017·Scientific Reports·Sumitra ShankarM Radhakrishna Pillai
Feb 26, 2011·Molecular BioSystems·Nataly Kravchenko-BalashaAlexander Levitzki
Feb 23, 2020·Beilstein Journal of Nanotechnology·Maxime DemazeauBarbara Lonetti
Oct 21, 2019·Biophysical Chemistry·Tayana Mazin TsuboneRosangela Itri
Sep 29, 2018·Bioorganic & Medicinal Chemistry·Sebastian A ThompsonCharles Michael Drain
Aug 10, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xue CaiRenjun Pei

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