Necrostatin-1 protects hippocampal neurons against ischemia/reperfusion injury via the RIP3/DAXX signaling pathway in rats

Neuroscience Letters
Rongli YangRuiguo Dong

Abstract

Global cerebral ischemia/reperfusion (I/R) induces selective neuronal injury in CA1 region of hippocampus, leading to severe impairment in behavior, learning and memory functions. However, the molecular mechanism underlying the processes was not elucidated clearly. RIP3 is a key molecular switch connecting apoptosis, necrosis and necroptosis. DAXX, as a novel substrate of RIP3, plays a vital role in ischemia-induced neuronal death. The aim of this study is to investigate the role and mechanism of RIP3/DAXX signaling pathway on neurons in CA1 region of the rat hippocampus after cerebral I/R. Global cerebral ischemia was induced by the method of four-vessel occlusion. RIP1 specific inhibitor Necrostatin-1 was administered by intracerebroventricular injection 1h before ischemia. Open-field, closed-field, and Morris water maze tests were performed respectively to examine the anxiety and cognitive behavior in each group. Hematoxylin and eosinstaining was used to examine the survival of hippocampal CA1 pyramidal neurons. Western blot or immunoprecipitation were carried to detect protein expression, phosphorylation, and interaction. We found that pre-treatment with Nec-1 protected locomotive ability, relieved anxiety behavior, and imp...Continue Reading

Citations

May 31, 2019·Cardiovascular Research·Prisca BoisguérinStéphanie Barrère-Lemaire
Aug 28, 2019·Cold Spring Harbor Perspectives in Biology·Benjamin J AndreoneJoseph W Lewcock
Feb 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Masanori Gomi NaitoJunying Yuan
Nov 14, 2019·Neural Regeneration Research·Li-Min GuoKun Xiong
Oct 18, 2020·Archives of Biochemistry and Biophysics·Suchan LiaoSiriporn C Chattipakorn
Nov 13, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Liyuan Cao, Wei Mu
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Jul 15, 2021·Oxidative Medicine and Cellular Longevity·Yue ZhouJinwen Ge
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Elena V MitroshinaMaria V Vedunova

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