Necrotic cell death in response to oxidant stress involves the activation of the apoptogenic caspase-8/bid pathway.

The Journal of Biological Chemistry
Xue WangAugustine M K Choi

Abstract

Human epithelial (A549) cells exposed to hyperoxia die by cellular necrosis. In the current study, we demonstrated the involvement of apoptogenic factors in epithelial cell necrosis in response to hyperoxia, including the formation of the Fas-related death-inducing signaling complex and initiation of mitochondria-dependent apoptotic pathways. We showed increased activation of both Bid and Bax in A549 cells subjected to hyperoxia. Bax activation involved a Bid-assisted conformational change. We discovered that the response to hyperoxia in vivo predominantly involved the activation of the Bid/caspase-8 pathway without apparent increases in Bax expression. Disruption of the Bid pathway by gene deletion protected against cell death in vivo and in vitro. Likewise, inhibition of caspase-8 by Flip also protected against cell death. Taken together, we have demonstrated the involvement of apoptogenic factors in epithelial cell responses to hyperoxia, despite a final outcome of cellular necrosis. We have, for the first time, identified a predominant role for the caspase-8/Bid pathway in signaling associated with hyperoxic lung injury and cell death in vivo and in vitro.

References

Oct 21, 1994·Cell·Z N Oltvai, S J Korsmeyer
May 1, 1994·Gut·M R Alison, C E Sarraf
Jun 21, 1996·The Journal of Biological Chemistry·J A KazzazS Horowitz
Feb 3, 1998·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyJ N Finkelstein
Feb 26, 1998·American Journal of Respiratory Cell and Molecular Biology·S A McGrath
Mar 21, 1998·Pharmacology & Therapeutics·M Raffray, G M Cohen
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y HuG Núñez
Apr 29, 1998·Annual Review of Physiology·G KroemerM Resche-Rigon
Aug 8, 1998·The International Journal of Biochemistry & Cell Biology·H J Brady, G Gil-Gómez
Oct 8, 1998·American Journal of Respiratory Cell and Molecular Biology·C BarazzoneP F Piguet
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyW M Maniscalco
Dec 1, 1998·International Archives of Allergy and Immunology·M FujitaN Hara
Mar 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·O MeilhacA Négre-Salvayre
Jun 25, 1999·Cell Death and Differentiation·C Kitanaka, Y Kuchino
Jul 29, 1999·Chest·S Horowitz
Sep 14, 1999·The American Journal of Physiology·E E Dupont-VersteegdenC A Peterson
Jan 11, 2000·Molecular and Cellular Biology·R EskesJ C Martinou
Feb 11, 2000·Annals of the New York Academy of Sciences·L L MantellJ A Kazzaz
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·G S PryhuberM H Nahm
Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·N S WardJ A Elias
Sep 26, 2000·Molecular Genetics and Metabolism·L L Mantell, P J Lee
Oct 18, 2000·The Journal of Biological Chemistry·W R FranekL L Mantell
Nov 21, 2000·The Journal of Experimental Medicine·J A HeibeinR C Bleackley
Jan 5, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·M A O'ReillyG S Pryhuber
Apr 21, 2001·Cellular and Molecular Life Sciences : CMLS·G DeneckerP Vandenabeele
Jun 21, 2001·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyP C Keng
Aug 18, 2001·American Journal of Respiratory Cell and Molecular Biology·S A McGrath-Morrow, J Stahl
Mar 6, 2002·The Journal of Biological Chemistry·G R Scott BudingerNavdeep S Chandel
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Humberto B JijonHoward G Parsons

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Citations

Dec 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·V A LesnikovH J Deeg
Apr 21, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Wenbin LiWei Liu
Feb 9, 2013·The International Journal of Biochemistry & Cell Biology·Xiaowei NieDaling Zhu
Oct 15, 2003·Free Radical Biology & Medicine·John RomashkoLin L Mantell
Nov 7, 2006·Nature Medicine·Vineet BhandariJack A Elias
Nov 23, 2006·Antioxidants & Redox Signaling·Stefan W RyterAugustine M K Choi
Feb 3, 2007·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Reinout A BemJob B M van Woensel
Apr 7, 2007·Anti-cancer Drugs·Kyohei TakahashiYukio Fujimoto
Jan 2, 2007·Current Opinion in Critical Care·William A Altemeier, Scott E Sinclair
Jul 16, 2004·Molecular and Cellular Biology·Punya ShrivastavaYvonne Janssen-Heininger
Apr 27, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yulia Y TyurinaValerian E Kagan
Sep 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Meng ZhangYang Jin
Oct 14, 2008·American Journal of Respiratory and Critical Care Medicine·Lynne A MurrayCory M Hogaboam
Jun 19, 2010·American Journal of Respiratory and Critical Care Medicine·Myung Hyun SohnJack A Elias
Oct 21, 2010·American Journal of Respiratory and Critical Care Medicine·G R Scott BudingerNavdeep S Chandel
Apr 19, 2011·American Journal of Respiratory and Critical Care Medicine·Rubin M TuderEric P Schmidt
Aug 17, 2004·American Journal of Respiratory Cell and Molecular Biology·Son V TruongGary W Hunninghake
Sep 10, 2005·American Journal of Respiratory Cell and Molecular Biology·Alessandra PaganoConstance Barazzone Argiroffo
Mar 8, 2008·American Journal of Respiratory Cell and Molecular Biology·Yang JinAugustine M K Choi
Jul 1, 2008·American Journal of Respiratory Cell and Molecular Biology·Quanfu MaoMonique E De Paepe
Nov 3, 2009·American Journal of Respiratory Cell and Molecular Biology·Rhonda J StaverskyMichael A O'Reilly
Nov 19, 2011·American Journal of Respiratory Cell and Molecular Biology·Akihiko TanakaAugustine M K Choi
Jan 10, 2012·American Journal of Respiratory Cell and Molecular Biology·Vineet BhandariJack A Elias
Apr 21, 2005·The Journal of Clinical Investigation·G R Scott Budinger, Jacob I Sznajder
Oct 14, 2005·Proceedings of the American Thoracic Society·Thomas R MartinGustavo Matute-Bello
Jan 25, 2013·Viruses·Elske van den BergReinout A Bem
Jul 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hong Pyo KimAugustine M K Choi
Apr 24, 2007·Free Radical Biology & Medicine·Xue WangStefan W Ryter
Jul 1, 2010·Journal of Immunotoxicology·Ashwini GoreLin L Mantell
Mar 15, 2006·Critical Reviews in Clinical Laboratory Sciences·Hellinida Thomadaki, Andreas Scorilas
Dec 7, 2010·Experimental Lung Research·Yu-Chieh M Wu, Michael A O'Reilly
Nov 10, 2004·Journal of Cellular Biochemistry·Lei YinBeatriz González-Flecha
May 1, 2010·Seminars in Fetal & Neonatal Medicine·Vineet Bhandari
Feb 17, 2010·Medicina intensiva·F Gordo-VidalI Salinas-Gabiña
Apr 9, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Atticus H HainsworthMichael J Whalen
Dec 24, 2008·Biochimica Et Biophysica Acta·Jeong Han KangOh-Shin Kwon

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