Abstract
This study was undertaken to determine the extent of necrotic cell death as a measure of neurologic injury in the preterm and near-term ovine fetal brain in response to intermittent umbilical cord occlusion (UCO) with severe, but limited hypoxia and no cumulative acidosis to ensure longer-term survival. Fetal sheep (control and experimental groups at 0.75 and 0.90 of gestation) were studied over 4 days with UCOs performed in the experimental group animals by complete inflation of an occluder cuff for 90 seconds every 30 minutes for 3 to 5 hours each day. Animals were then euthanized and the fetal brain perfusion-fixed and prepared for subsequent histology and assessment of necrotic cell injury by using standard staining with hematoxylin and eosin (H&E), and with a novel fluorescent marker, Fluoro-Jade B, that targets degenerating neurons. In both preterm and near-term animal groups, UCO caused a large decline in arterial Po(2) (to approximately 7 mm Hg), a modest decline in pH (to approximately 7.30), and a modest rise in Pco(2) (to approximately 61 mm Hg) (all P<.01), but with a return to control values after the occluder release and no cumulative acidosis over each day of study. Overall, very low levels of H&E-stained necroti...Continue Reading
References
Nov 1, 1992·American Journal of Obstetrics and Gynecology·E C MallardP D Gluckman
Oct 1, 1991·American Journal of Obstetrics and Gynecology·I A HoskinsB K Young
Jul 1, 1990·Teratology·T E WhiteR K Miller
Aug 5, 1987·Neuroscience Letters·H HagbergM Thordstein
Aug 1, 1988·American Journal of Obstetrics and Gynecology·J F ClappL I Mann
Oct 1, 1986·American Journal of Obstetrics and Gynecology·A AnyaegbunamO Langer
Mar 1, 1984·American Journal of Obstetrics and Gynecology·J A LowE J Karchmar
Apr 1, 1982·Journal of Neurosurgery·J Astrup
Jun 1, 1995·Pediatric Research·E C MallardP D Gluckman
Jan 1, 1993·American Journal of Obstetrics and Gynecology·G S DawesT Wheeler
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P W HochachkaS C Land
Mar 1, 1997·American Journal of Obstetrics and Gynecology·H KeunenT H Hasaart
Sep 10, 1998·American Journal of Obstetrics and Gynecology·K B Nelson, J K Grether
Apr 17, 1999·Journal of the Society for Gynecologic Investigation·L R GreenB S Richardson
Nov 11, 1999·Journal of the Society for Gynecologic Investigation·J F ClappS Simonean
Sep 7, 2000·The Journal of Endocrinology·L R GreenV K Han
Sep 3, 2004·American Journal of Obstetrics and Gynecology·Elizabeth RochaBryan Richardson
Citations
Jun 30, 2012·PloS One·Andrew P ProutBryan S Richardson
Nov 14, 2013·PloS One·Margie Castillo-MelendezDavid W Walker
Feb 5, 2016·Journal of Neuroinflammation·Daan R M G OpheldersTim G A M Wolfs
Sep 25, 2007·Brain Research·Victor M PulgarJorge P Figueroa
Nov 6, 2009·American Journal of Obstetrics and Gynecology·Andrew P ProutBryan S Richardson
May 12, 2016·Journal of Neuroinflammation·M G FraschB S Richardson
Dec 4, 2008·Reproductive Sciences·Hidekazu NishigoriBryan S Richardson
Jun 25, 2013·Reproductive Sciences·Joepe J KaandorpDino A Giussani
Oct 1, 2011·Reproductive Sciences·Jennifer A ThompsonTimothy R H Regnault
Dec 26, 2015·Reproductive Sciences·Alex XuBryan S Richardson
Oct 6, 2005·Journal of the Society for Gynecologic Investigation·Laura NardoAlan D Bocking
Apr 5, 2014·Reproductive Sciences·Marie J CzikkBryan S Richardson
Jul 20, 2007·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology
Oct 2, 2007·Australian Veterinary Journal·F DutraG Banchero
Mar 29, 2013·The Journal of Toxicological Sciences·Diana CardonaFernando Sánchez-Santed
Nov 14, 2018·Pediatric Research·Andrew GhalyBryan S Richardson
May 3, 2011·Journal of Animal Science·F Dutra, G Banchero