Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury

American Journal of Physiology. Lung Cellular and Molecular Physiology
Ziyan ZhuRichard D Ye

Abstract

Lung infections are major causes of acute lung injury (ALI), with limited effective treatment available. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is an essential adaptor regulating Toll-like receptors (TLRs). We recently identified Cullin-5 (Cul-5) as a prominent component in the regulation of TRAF6 polyubiquitination, but its physiological significance in ALI has not been explored. In this study, we investigated the potential role of Cul-5 in regulating ALI using mice receiving intratracheal instillation of LPS. We observed that Cul-5-deficient mice displayed reduced lung injury compared with wild-type mice as evidenced by histological analysis, alveolar neutrophil infiltration, and lung liquid accumulation. In addition, inflammatory cytokine expression in bronchoalveolar lavage fluid and lung tissue was also markedly reduced in LPS-treated Cul-5-deficient mice. Interestingly, intratracheal adoptive transfer of Cul-5+/- but not Cul-5+/+ macrophages attenuated neutrophil recruitment, alveolar inflammation, and loss of barrier function in LPS-challenged wild-type mice. Finally, we demonstrated that Cul-5 neddylation following LPS exposure induced Cul-5 and TRAF6 interaction and, thereby, TFAR6 polyubiquitinatio...Continue Reading

References

Oct 1, 1992·The British Journal of Surgery·C R Welbourn, Y Young
Apr 12, 2003·Critical Care Medicine·Edward Abraham
Oct 18, 2003·Cytokine & Growth Factor Reviews·Richard B GoodmanMichael A Matthay
Oct 28, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hao Wu, Joseph R Arron
Jan 31, 2004·Seminars in Immunology·Kiyoshi Takeda, Shizuo Akira
Nov 24, 2004·Infection and Immunity·Samithamby JeyaseelanG Scott Worthen
Oct 21, 2005·The New England Journal of Medicine·Gordon D RubenfeldLeonard D Hudson
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Jul 16, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gustavo Matute-BelloThomas R Martin
Oct 15, 2008·Molecular Cell·Anjanabha Saha, Raymond J Deshaies
Apr 11, 2009·Nature·Teresa A SoucySteven P Langston
Jun 30, 2009·Nature Immunology·Maziar DivangahiHeinz G Remold
Jan 16, 2010·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Elizabeth R Johnson, Michael A Matthay
Feb 18, 2010·Proceedings of the American Thoracic Society·Curtis H WeissManu Jain
Mar 20, 2010·Cold Spring Harbor Perspectives in Biology·Ingrid E Wertz, Vishva M Dixit
May 3, 2011·American Journal of Respiratory Cell and Molecular Biology·Gustavo Matute-BelloUNKNOWN Acute Lung Injury in Animals Study Group
May 11, 2011·Genome Biology·Antonio SarikasZhen-Qiang Pan
Mar 8, 2012·Chest·István VadászJacob I Sznajder
Mar 21, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jing ZhaoYutong Zhao
Nov 16, 2013·EMBO Reports·John R LydeardJ Wade Harper
Feb 11, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Neil R AggarwalFranco R D'Alessio
Apr 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lei SunRichard D Ye
Jun 24, 2015·Journal of Molecular Cell Biology·Feng QianJohn W Christman
Jun 28, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaohui FangMichael A Matthay
Jul 15, 2015·Science Translational Medicine·Tiffany A CoonBill B Chen
Aug 9, 2015·Biochimie·Isabelle LamsoulPierre G Lutz
May 11, 2016·The Journal of Experimental Medicine·Travis LearBill B Chen
May 29, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ziyan ZhuRichard D Ye

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Citations

Mar 21, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rui HaoLei Sun
Aug 5, 2020·Immunity, Inflammation and Disease·Yun Lu, Xuguang Yang
Mar 12, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Badreldin H AliAbderrahim Nemmar
Jul 20, 2019·The European Respiratory Journal·Yusuke TakeiMasakazu Ichinose
Oct 8, 2020·The FEBS Journal·Tao Zou, Jiyan Zhang
Jun 25, 2021·Frontiers in Cell and Developmental Biology·Yanyu JiangLijun Jia

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Methods Mentioned

BETA
ubiquitination
gene-trapping
lavage
PCR
ELISA
Assay
Transfection
gene trapping
genotyping
bronchoalveolar lavage

Software Mentioned

Prism

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