Neem leaf glycoprotein reverses tumor-induced and age-associated thymic involution to maintain peripheral CD8+ T cell pool.

Immunotherapy
Ipsita GuhaAnamika Bose

Abstract

Aim: As tumor causes atrophy in the thymus to target effector-T cells, this study is aimed to decipher the efficacy of neem leaf glycoprotein (NLGP) in tumor- and age-associated thymic atrophy. Materials & methods: Different thymus parameters were studied using flow cytometry, reverse transcriptase PCR and immunocyto-/histochemistry in murine melanoma and sarcoma models. Results: Longitudinal NLGP therapy in tumor hosts show tumor-reduction along with significant normalization of thymic alterations. NLGP downregulates intrathymic IL-10, which eventually promotes Notch1 to rescue blockade in CD25+CD44+c-Kit+DN2 to CD25+CD44-c-Kit-DN3 transition in T cell maturation and suppress Ikaros/IRF8/Pu.1 to prevent DN2-T to DC differentiation in tumor hosts. The CD5intTCRαβhigh DP3 population was also increased to endorse CD8+ T cell generation. Conclusion: NLGP rescues tumor-induced altered thymic events to generate more effector T cells to restrain tumor.

References

Jul 1, 1991·Immunology Today·S R CardingK Bottomly
Nov 1, 1994·The Journal of Experimental Medicine·J J PeschonB L Davison
Aug 17, 1999·Journal of Leukocyte Biology·T Brocker
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B AdkinsD M Lopez
Mar 4, 2003·Nature Medicine·Thomas A Waldmann
Apr 30, 2003·Nature Immunology·Yuichi WakabayashiRyo Kominami
Jul 3, 2003·The Journal of Antimicrobial Chemotherapy·Rafael de la Rosa, Manuel Leal
May 19, 2004·Immunology·Gloria SoldevilaEduardo García-Zepeda
Aug 11, 2004·The Journal of Experimental Medicine·Tomoo UenoYousuke Takahama
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael A CannarileThomas Brocker
Feb 24, 2006·Nature Reviews. Immunology·Yousuke Takahama
Sep 5, 2006·Nature Immunology·Roberto BonasioUlrich H von Andrian
Oct 28, 2006·Toxicologic Pathology·Susan A Elmore
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin A SchwarzAvinash Bhandoola
May 18, 2007·Annual Review of Cell and Developmental Biology·Maria Ciofani, Juan Carlos Zúñiga-Pflücker
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Chace TydellEllen V Rothenberg
Dec 22, 2007·Nature Reviews. Immunology·Ellen V RothenbergMary A Yui
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ayelet Laronne-Bar-OnNechama Haran-Ghera
Jun 10, 2009·Age·Sara Ferrando-MartínezManuel Leal
Dec 8, 2010·Immunology and Cell Biology·Mark D BuntingShaun R McColl
Aug 17, 2011·Immunotherapy·Tathagata ChakrabortyRathindranath Baral
Jun 5, 2012·Cold Spring Harbor Perspectives in Biology·Yan Xing, Kristin A Hogquist
Jun 21, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anamika BoseSubrata Majumdar
Nov 8, 2013·Immunologic Research·Roberto Carrio, Diana M Lopez
Apr 22, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Divya K Shah, Juan Carlos Zúñiga-Pflücker
Apr 29, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charles Sinclair, Benedict Seddon
Jun 6, 2014·Journal of Immunology Research·Glauben LandskronMarcela A Hermoso
Aug 17, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dorina Avram, Danielle Califano
Feb 16, 2015·Oncotarget·Kevin Van der JeughtKarine Breckpot
Jan 15, 2016·European Journal of Immunology·Wooseok Seo, Ichiro Taniuchi
Jan 23, 2016·Advances in Immunology·Ellen V RothenbergAmeya Champhekar
Jan 31, 2016·Cellular and Molecular Life Sciences : CMLS·Takeshi Nitta, Harumi Suzuki

❮ Previous
Next ❯

Citations

Jun 12, 2021·Frontiers in Immunology·Mingli LuoXi Sun

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
FACS
flow-cytometry
flow cytometry
ELISA

Software Mentioned

FlowJo
NLGP
INSTAT3
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.