Nef can enhance the infectivity of receptor-pseudotyped human immunodeficiency virus type 1 particles.

Journal of Virology
Massimo PizzatoHeinrich G Göttlinger

Abstract

Nef is an accessory protein of human immunodeficiency virus type 1 (HIV-1) that enhances the infectivity of progeny virions when expressed in virus-producing cells. The requirement for Nef for optimal infectivity is, at least in part, determined by the envelope (Env) glycoprotein, because it can be eliminated by pseudotyping HIV-1 particles with pH-dependent Env proteins. To investigate the role of Env in the function of Nef, we have examined the effect of Nef on the infectivity of Env-deficient HIV-1 particles pseudotyped with viral receptors for cells expressing cognate Env proteins. We found that Nef significantly enhances the infectivity of CD4-chemokine receptor pseudotypes for cells expressing HIV-1 Env. Nef also increased the infectivity of HIV-1 particles pseudotyped with Tva, the receptor for subgroup A Rous sarcoma virus (RSV-A), even though Nef had no effect if the pH-dependent Env protein of RSV-A was used for pseudotyping. However, Nef does not always enhance viral infectivity if the normal orientation of the Env-receptor interaction is reversed, because the entry of Env-deficient HIV-1 into cells expressing the vesicular stomatitis virus G protein was unaffected by Nef. Together, our results demonstrate that the p...Continue Reading

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Citations

May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Massimo Pizzato
Nov 7, 2008·Journal of Virology·Nadine LaguetteStéphane Basmaciogullari
Feb 26, 2010·Journal of Virology·Elena SokolskajaJeremy Luban
Jul 31, 2012·Future Virology·Shailendra K SaxenaMadhavan Pn Nair
Jul 3, 2010·Molecular Aspects of Medicine·Nadine LaguetteStéphane Basmaciogullari
Sep 23, 2009·Microbes and Infection·Abhay JereMasako Nomaguchi
Sep 15, 2009·Reviews in Medical Virology·David WarrilowDavid Harrich
Sep 30, 2015·Nature·Annachiara RosaMassimo Pizzato
Jun 7, 2014·Frontiers in Microbiology·Stéphane Basmaciogullari, Massimo Pizzato
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Ajit ChandeMassimo Pizzato
Mar 10, 2017·Journal of Virology·Xianfeng ZhangYong-Hui Zheng
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq
Sep 29, 2018·Annual Review of Virology·Claudia FirritoMassimo Pizzato
Feb 10, 2017·The Journal of Biological Chemistry·Chetan SoodGregory B Melikyan

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