PMID: 7541845Aug 1, 1995Paper

Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis

Journal of Virology
C Aiken, D Trono

Abstract

The Nef protein of human immunodeficiency virus type 1 (HIV-1) stimulates viral infectivity. The mechanism of this phenotype was investigated. Viruses containing disrupted nef genes were 4 to 40 times less infectious than wild-type HIV-1 in a single-round infection. The Nef-mediated stimulation HIV-1 infectivity was dependent on the association of Nef with the plasma membrane and could be observed when Nef was provided in trans in the virus producer but not target cells. The impaired infectiousness of nef-defective (delta Nef) virions was observed whether or not CD4 was present in either of these cells. Furthermore, it was independent of the mode of viral entry, since it was not rescued by pseudotyping Env- HIV-1 virions with the amphotropic murine leukemia virus envelope glycoproteins. As predicted from this result, wild-type and delta Nef virions entered cells with equal efficiencies. However, despite their normal content in viral genomic RNA and reverse transcriptase activity, delta Nef viruses were limited in their ability to perform reverse transcription once internalized in several cell types, including peripheral blood lymphocytes. Since Nef does not appear to be abundant in virions, these results suggest that Nef acts i...Continue Reading

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Citations

Nov 20, 1996·AIDS Research and Human Retroviruses·R GeleziunasW C Greene
May 23, 1997·The Journal of Biological Chemistry·L X LiuS Benichou
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·P GallayD Trono
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Feb 18, 2004·The Journal of Biological Chemistry·Bastien MangeatDidier Trono
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