Negative autoregulation of the organizer-specific homeobox gene goosecoid.

The Journal of Biological Chemistry
V DanilovH Steinbeisser

Abstract

The homeobox gene goosecoid has been implicated to play a central role in the Spemann organizer tissue of the vertebrate embryo. Misexpression of goosecoid on the ventral side of a Xenopus laevis gastrula embryo was shown to result in a partial duplication of the primary body axis, reminiscent of the Spemann organizer graft. Normal embryonic development thus requires tight temporal and spatial control of genes instrumental for organizer function. In the present study we investigated the transcriptional control of goosecoid gene expression. Sequence analysis of the mouse and human promoter region revealed the presence of two palindromic binding elements for homeobox genes of the prd type to which goosecoid belongs. We show that Goosecoid protein can bind to these sites in vitro. By using reporter gene constructs of the human and mouse promoter, we demonstrate that Goosecoid can act as a repressor of its own promoter activity in transient co-transfection experiments in mouse P19 cells and in Xenopus embryos. Autorepression depends on the presence of the homeodomain and is mediated through the prd element more proximal to the transcriptional start site. Our results suggest a role for goosecoid in restricting organizer activity in ...Continue Reading

References

Oct 11, 1992·Nucleic Acids Research·M J KernS S Potter
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·H BastianJ C Izpisúa-Belmonte
Jul 12, 1991·Science·B BlumbergK W Cho
Jan 1, 1995·Current Biology : CB·D S Wilson, C Desplan
Mar 30, 1995·Nature·W Shawlot, R R Behringer
Feb 24, 1995·The Journal of Biological Chemistry·P Q ThomasP D Rathjen
Nov 1, 1993·Genes & Development·D WilsonC Desplan

❮ Previous
Next ❯

Citations

Aug 10, 2000·Journal of Neuroscience Research·R AwatramaniJ Kamholz
Mar 22, 2001·The Plant Journal : for Cell and Molecular Biology·M OhgishiT Aoyama
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Toshiyasu GotoMakoto Asashima
Dec 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kimberly A HartwellRobert A Weinberg
Mar 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya KoideKen W Y Cho
Mar 14, 2008·Experimental Cell Research·Luisa IzziLiliana Attisano
May 15, 2012·Developmental Biology·Lloyd A PereiraAndrew G Elefanty
Oct 20, 2007·Acta histochemica·Hung Ping ShihChrissa Kioussi
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Husniye KantarciBruce B Riley
May 5, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C C ZhuM Blum
Mar 10, 2016·Development·Emese GazdagGert Jan C Veenstra
May 26, 2007·The EMBO Journal·Veronika SanderE M De Robertis
Nov 28, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jeremy Green
May 7, 2004·Development·Craig T MillerCharles B Kimmel
Aug 22, 2008·Development·Daniel L GarauletErnesto Sánchez-Herrero

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.