Negative dielectrophoretic patterning with different cell types

Biosensors & Bioelectronics
Masato SuzukiTomokazu Matsue

Abstract

In this paper, a novel method for patterning different cell types based on negative dielectrophoresis (n-DEP), without any special pretreatment of a culture slide, has been described. An interdigitated array (IDA) electrode with four independent microelectrode subunits was fabricated with indium-tin-oxide (ITO) and used as a template to form cellular micropatterns. A suspension of C2C12 cells was introduced into the patterning device between the upper slide and the bottom IDA. In the present system, the n-DEP force is induced by applying an ac voltage (typically 12V(pp), 1MHz) to direct cells toward a weaker region of electric field strength. The cells aligned above one of the bands of IDA within 1min since the aligned areas on the slide were regions with the lower electric field. The application of an ac voltage for 5min allows the cells to adsorb onto the cell culture slide. After removing excess cells, the second cell type was patterned in lines using the same method as with the first set of cells. Periodic and alternate cell lines incorporating two cell types were also fabricated by changing the ac voltage mode. A second cell type was introduced into the device and guided to other areas to form a different pattern. The desc...Continue Reading

References

Dec 30, 1999·Biomaterials·R S KaneG M Whitesides
Jun 10, 2004·Electrophoresis·Blanca H Lapizco-EncinasYolanda Fintschenko
Dec 1, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Masato SuzukiTomokazu Matsue
Mar 5, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Hirokazu KajiMatsuhiko Nishizawa
Mar 15, 2005·Biomacromolecules·Jun-Ichi EdahiroYasuo Yoshimi
Feb 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Masato SuzukiTomokazu Matsue

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Citations

Jan 17, 2012·Analytical Chemistry·Mehdi JavanmardRonald W Davis
Sep 9, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Munehiro YamaguchiKazutoshi Gohara
Mar 15, 2011·Immunotherapy·Masahiro Tomita, Kanta Tsumoto
Oct 13, 2012·Annual Review of Cell and Developmental Biology·Gregory H UnderhillSangeeta N Bhatia
Dec 11, 2013·Tissue Engineering. Part B, Reviews·Serge OstrovidovAli Khademhosseini
Oct 31, 2012·Science China. Life Sciences·Peiyuan GongXingyu Jiang
Jul 27, 2010·Biochimica Et Biophysica Acta·Hirokazu KajiAli Khademhosseini
Dec 17, 2009·Colloids and Surfaces. B, Biointerfaces·Jinbo GanZhongkui Wu
Apr 14, 2018·Tissue Engineering. Part B, Reviews·Molly N Pantelic, Lisa M Larkin
Jun 7, 2018·Lab on a Chip·Dohyun ParkNoo Li Jeon
Sep 17, 2011·Electrophoresis·Barbaros Cetin, Dongqing Li
Jul 12, 2013·Lab on a Chip·Feng GuoTony Jun Huang
Apr 23, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tomoyuki YasukawaFumio Mizutani
Apr 16, 2020·Advanced Biosystems·Kosuke InoHitoshi Shiku
Jun 19, 2009·Biomacromolecules·Terry R ColbourneLaurent Kreplak
Jun 21, 2014·Analytical Chemistry·Yuki YoshimuraTomoyuki Yasukawa
Aug 15, 2021·Cell and Tissue Research·Deming JiangPing Wang

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