Negative effects of a high tumour necrosis factor-α concentration on human gingival mesenchymal stem cell trophism: the use of natural compounds as modulatory agents

Stem Cell Research & Therapy
Chiara GiacomelliClaudia Martini

Abstract

Adult mesenchymal stem cells (MSCs) play a crucial role in the maintenance of tissue homeostasis and in regenerative processes. Among the different MSC types, the gingiva-derived mesenchymal stem cells (GMSCs) have arisen as a promising tool to promote the repair of damaged tissues secreting trophic mediators that affect different types of cells involved in regenerative processes. Tumour necrosis factor (TNF)-α is one of the key mediators of inflammation that could affect tissue regenerative processes and modify the MSC properties in in-vitro applications. To date, no data have been reported on the effects of TNF-α on GMSC trophic activities and how its modulation with anti-inflammatory agents from natural sources could modulate the GMSC properties. GMSCs were isolated and characterized from healthy subjects. The effects of TNF-α were evaluated on GMSCs and on the well-being of endothelial cells. The secretion of cytokines was measured and related to the modification of GMSC-endothelial cell communication using a conditioned-medium method. The ability to modify the inflammatory response was evaluated in the presence of Ribes nigrum bud extract (RBE). TNF-α differently affected GMSC proliferation and the expression of inflammato...Continue Reading

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Citations

Aug 15, 2018·International Journal of Molecular Sciences·Yi-Heng LinYen-Hua Huang
Oct 22, 2020·International Journal of Molecular Sciences·Deborah PietrobonoMaria Letizia Trincavelli
Dec 19, 2020·Journal of Enzyme Inhibition and Medicinal Chemistry·Elisabetta BarresiSabrina Taliani
Feb 8, 2020·Journal of Natural Products·Anna Maria IannuzziAlessandra Braca
May 15, 2021·Integrative Biology : Quantitative Biosciences From Nano to Macro·Johanna S DuttonNancy L Allbritton

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Methods Mentioned

BETA
PCR
electrophoresis
Assay
enzyme-linked immunosorbent assay
ELISA
biopsies

Software Mentioned

ImageJ
GraphPad
Image J
Xcalibur
STRING ( Search Tool for the Retrieval
GraphPad Prism

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