Negative growth control of renal cell carcinoma cell by connexin 32: possible involvement of Her-2

Molecular Carcinogenesis
Eriko FujimotoT Yano

Abstract

Connexin (Cx) genes have negative growth effects on tumor cells with certain cell specificity. We have previously reported that Cx32 is specifically downregulated in human renal cell carcinoma cell (RCC) lines as well as cancerous regions of kidneys and that the Cx is expressed in the progenitor cells of the carcinoma. However, the precise role of Cx32 in growth control of RCC cells remains unknown. In this study, we examined whether Cx32 could act in growth control against a human RCC cell, Caki-2 cell. In order to estimate the cell growth control, we established Caki-2 cells that have stable expression of Cx32 genes. Cx32 expression in Caki-2 cells induced contact inhibition of growth and reduced anchorage-independent growth ability, but did not significantly affect lag phase growth rates. This growth control by Cx32 was dependent on the inhibition of the cell-cycle transition from G1 to S phase at high cell density, and the inhibition of the cell-cycle transition related to the suppression of Her-2 activation. Furthermore, the suppression of Cx32 expression in Caki-2 cells by short interfering RNA induced the activation of Her-2. These data suggest that Cx32 has negative growth control of Caki-2 cells, partly due to the inhi...Continue Reading

References

Jan 21, 1977·Science·I SimpsonW R Loewenstein
Oct 1, 1991·The Journal of Cell Biology·K A StaufferN Unwin
Jun 1, 1990·Molecular and Cellular Biology·E Di MarcoP P Di Fiore
Jan 1, 1990·Teratogenesis, Carcinogenesis, and Mutagenesis·D J Fitzgerald, H Yamasaki
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J C SáezM V Bennett
Sep 1, 1986·The Journal of Cell Biology·N M Kumar, N B Gilula
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L BrissetteG P Dotto
Oct 1, 1995·Current Opinion in Cell Biology·D L Paul
Feb 9, 1996·Cell·N M Kumar, N B Gilula
Jun 1, 1996·Carcinogenesis·H Yamasaki, C C Naus
Dec 6, 1996·Science·C J Sherr
Mar 21, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D J Riese, D F Stern
Dec 3, 1999·Biochemical and Biophysical Research Communications·T KojimaD C Spray
Dec 14, 2001·The Journal of Biological Chemistry·Lilian I PlotkinTeresita Bellido
Jul 11, 2002·Biological Chemistry·Klaus WilleckeGoran Söhl
Apr 12, 2003·American Journal of Nephrology·Ayako HiraiHiroshi Yamasaki

❮ Previous
Next ❯

Citations

Feb 7, 2009·Cell Death and Differentiation·E DecrockL Leybaert
Oct 7, 2008·Antioxidants & Redox Signaling·Laurent CronierMarc Mesnil
Aug 31, 2010·Biochimica Et Biophysica Acta·Mathieu VinkenVera Rogiers
Oct 30, 2007·Cellular & Molecular Biology Letters·Jarosław Czyz
Dec 31, 2014·Cell and Tissue Research·Trond Aasen
Jul 2, 2011·Biochimica Et Biophysica Acta·Mathieu VinkenVera Rogiers
Feb 9, 2005·Journal of Pharmacological Sciences·Eriko FujimotoKoichi Ueno
Apr 29, 2016·Antioxidants & Redox Signaling·Yeon Ju KimYun-Hoon Choung
Nov 6, 2013·Infection and Immunity·Jana N RadinTimothy L Cover
Dec 23, 2006·Molecular Carcinogenesis·Hiromi SatoTomohiro Yano
Dec 7, 2007·Molecular Carcinogenesis·Hiromi SatoTomohiro Yano
Feb 22, 2012·Nature Reviews. Urology·Jarosław CzyżZbigniew Madeja
Nov 29, 2007·American Journal of Physiology. Endocrinology and Metabolism·Gaëlle ProstBernard Rousset
Feb 15, 2007·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Eriko FujimotoKoichi Ueno
Dec 20, 2018·Acta Biochimica Et Biophysica Sinica·Zheng ZhuJianlin Yuan
May 8, 2007·Progress in Biophysics and Molecular Biology·Michael Levin
Dec 17, 2020·Biomolecules·Erin E Mulkearns-HubertJustin D Lathia

❮ Previous
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