PMID: 507Nov 7, 1975

Negative potential level in the outer layer of the toad skin

The Journal of Membrane Biology
M A Nunes, F L Vieira

Abstract

The isolated skin of the toad Bufo marinus ictericus when impaled from the outer surface by glass microelectrodes filled with 3 M KCl shows a voltage profile which is a continuous function of the depth of impalement. The superficial intraepithelial potential difference measured with reference to the external solution (PDi) is negative with NaCl-Ringer's solution on both sides of the skin, displaying a minimum of -26.7+/-3.6 mV at 6+/-2 mum. Null value is obtained at 19+/-3 mum, with positive values for deeper impalements. Indications of cell impalements (abrupt voltage and resistance jumps) were frequently observed at sites deeper than 25 mum from the outer surface. Measurements of the electrical resistance between the microelectrode and the external solution, made with single- and double-barreled microelectrodes, showed great discrepancies, which may be attributed to distinct pathways of different resistances in the stratum corneum. PDi measured at a depth of 5 mum was a logarithmic function of Na2SO4 or K2SO4 concentration in the external solution, increasing in negativity with a reduction in concentration. Substitution of Na by K in the external solution had only minor effects on PDi. Acidification of the external solution f...Continue Reading

References

Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A Martínez-Palomo, D Erlij
Aug 1, 1971·The Journal of Cell Biology·A Martínez-PalomoH Bracho
May 1, 1972·The Journal of General Physiology·L J Mandel, P F Curran
Jul 1, 1970·The American Journal of Physiology·T L DavisW S Rehm
Apr 1, 1970·Experimental Eye Research·J P Fee, H F Edelhauser
Jan 1, 1970·Pflügers Archiv : European journal of physiology·F RawlinsG WHITTEMBURY
Dec 1, 1970·The American Journal of Physiology·N Akaike, M Hori
Mar 14, 1966·Bibliotek for laeger·T K CHOWDHURY, F M SNELL
Jul 1, 1966·The Journal of General Physiology·T U BiberP F Curran
Jun 1, 1966·Journal of Cellular Physiology·D W Martin, P F Curran
Jul 1, 1957·Acta Physiologica Scandinavica·L ENGBAEK, T HOSHIKO
Jun 2, 1958·Acta Physiologica Scandinavica·V KOEFOED-JOHNSEN, H H USSING
Mar 1, 1964·The Journal of General Physiology·B D LINDLEY, T HOSHIKO
Mar 1, 1964·The Journal of General Physiology·G WHITTEMBURY
Jun 1, 1964·Experimental Eye Research·Y KIKKAWA
Mar 29, 1965·Biochimica Et Biophysica Acta·T K CHOWDHURY, F M SNELL
Mar 1, 1965·The Journal of General Physiology·M CEREIJIDO, P F Curran
Nov 1, 1961·Acta Physiologica Scandinavica·E A MACROBBIE, H H USSING
Dec 1, 1949·Journal of Cellular Physiology·G N Ling, R W GERARD
Dec 1, 1972·The Journal of Membrane Biology·D Erlij, A Martínez-Palomo

Citations

Sep 30, 1976·Pflügers Archiv : European journal of physiology·W Nagel
Dec 12, 1979·The Journal of Membrane Biology·W Nagel
Apr 26, 1978·The Journal of Membrane Biology·F L Vieira, M I Onuchic
May 1, 1977·The Journal of General Physiology·S I Helman, R S Fisher
Jan 1, 1976·The Journal of Membrane Biology·F L VieiraL Cury

Related Concepts

Metazoa
Bufo marinus
Electric Conductivity
Electric Stimulation Technique
Epithelial Cells
Mesothelium
Hydrogen-Ion Concentration
Resting Potentials
Microelectrodes
Potassium

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