Negative-Pressure Wound Therapy Promotes Wound Healing by Enhancing Angiogenesis Through Suppression of NLRX1 via miR-195 Upregulation

The International Journal of Lower Extremity Wounds
Yu LiuJianda Zhou

Abstract

Negative-pressure wound therapy (NPWT) is one of the most advanced therapeutic methods in the treatment of various hard-to-heal acute and refractory chronic wounds. Recent emerging evidence points to a role of the microRNA-mediated regulation of angiogenesis in ischemic tissues, and a series of microRNAs associated with angiogenesis have been successively identified. In this study, we found that miR-195 expression was significantly upregulated and the microvessel density (MVD) was increased in granulation tissue collected 7 days after NPWT compared with those in the pre-NPWT tissue. Moreover, the expression of NLRX1, the potential target gene of miR-195, was down-regulated in post-NPWT compared with that in pre-NPWT tissue. Significant negative correlations were detected between miR-195 and NLRX1 expression levels ( r = -.856, P < .001) and between NLRX1 expression and MVD ( r = -.618, P < .05), whereas miR-195 expression was positively correlated with MVD in the granulation tissue ( r = .630, P < .05). In summary, NPWT may suppress NLRX1 expression through the upregulation of miR-195 expression, thus efficaciously promoting angiogenesis in the granulation tissue to enhance wound healing.

References

Dec 16, 1992·Journal of the National Cancer Institute·N WeidnerG Gasparini
Aug 6, 2002·Breast Cancer Research and Treatment·Arihiro ShibataHisao Seo
Jan 30, 2003·RNA·Mariana Lagos-QuintanaThomas Tuschl
Apr 28, 2007·Cell Death and Differentiation·S P Tabruyn, A W Griffioen
Mar 27, 2012·Immunity·Tsan Sam Xiao, Jenny P-Y Ting
Jul 18, 2012·Cardiovascular Research·Yung-Song WangSuh-Hang Hank Juo
Mar 8, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ruizhi WangShi-Mei Zhuang
Oct 11, 2014·Advances in Wound Care·Luca Lancerotto, Dennis P Orgill
Oct 11, 2014·Advances in Wound Care·Kelly E Johnson, Traci A Wilgus
Jul 4, 2017·Current Cancer Drug Targets·Ankit TiwariManjusha Dixit
Apr 19, 2018·The Journal of International Medical Research·Yi WangXiong-Wen Lü

❮ Previous
Next ❯

Citations

Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Tiia Snäkä, Nicolas Fasel
Jan 6, 2022·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Yash MantriJesse V Jokerst

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
biopsies

Software Mentioned

TargetScan
Quantity One Image Analysis
PicTar
MiRanda

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.