PMID: 9555083Jun 20, 1998Paper

Negative regulation of Apo A-I gene expression by retinoic acid in rat hepatocytes maintained in a coculture system

Biochimica Et Biophysica Acta
L BerthouA Guillouzo

Abstract

Rat hepatocytes cocultured with rat liver epithelial cells (RLEC) were used to investigate the influence of all-trans retinoic acid (RA) on the regulation of apolipoproteins (Apo) A-I and A-II gene expression, the major protein constituent of high-density lipoproteins. In contrast to rat hepatocytes in conventional primary culture, Apo A-I and Apo A-II gene expression remained high and stable for several days in parenchymal cells in coculture. Treatment of cocultured rat hepatocytes with RA resulted in a specific decrease in Apo A-I mRNA levels whereas no marked difference in Apo A-II mRNA levels was observed. Such a negative effect of RA was already detected as early as 2 days of treatment and was effective for the entire experimental period (6 days). As controls, RARbeta mRNA levels increased whereas those of GAPDH mRNA were not affected by the RA treatment. The decrease in Apo A-I mRNA levels was associated with lower amounts of Apo A-I secreted in the culture medium within day 1 of treatment. This effect required active transcription and protein synthesis. These results show that, contrary to primary pure hepatocyte cultures and hepatoma cell lines, cocultures of rat hepatocytes reproduce the in vivo results suggesting that...Continue Reading

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Citations

Mar 8, 2014·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Arshag D Mooradian, Michael J Haas
Nov 22, 2011·Maternal & Child Nutrition·Larissa Queiroz de LiraRoberto Dimenstein
Aug 30, 2011·Biomaterials·Simona SalernoLoredana De Bartolo
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Oct 6, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Emilie GrenierEmile Levy

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