Negative regulation of beta enolase gene transcription in embryonic muscle is dependent upon a zinc finger factor that binds to the G-rich box within the muscle-specific enhancer.

The Journal of Biological Chemistry
R PassantinoA Giallongo

Abstract

We have previously identified a muscle-specific enhancer within the first intron of the human beta enolase gene. Present in this enhancer are an A/T-rich box that binds MEF-2 protein(s) and a G-rich box (AGTGGGGGAGGGGGCTGCG) that interacts with ubiquitously expressed factors. Both elements are required for tissue-specific expression of the gene in skeletal muscle cells. Here, we report the identification and characterization of a Kruppel-like zinc finger protein, termed beta enolase repressor factor 1, that binds in a sequence-specific manner to the G-rich box and functions as a repressor of the beta enolase gene transcription in transient transfection assays. Using fusion polypeptides of beta enolase repressor factor 1 and the yeast GAL4 DNA-binding domain, we have identified an amino-terminal region responsible for the transcriptional repression activity, whereas a carboxyl-terminal region was shown to contain a potential transcriptional activation domain. The expression of this protein decreases in developing skeletal muscles, correlating with lack of binding activity in nuclear extract from adult skeletal tissue, in which novel binding activities have been detected. These results suggest that in addition to the identified f...Continue Reading

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Citations

May 29, 2003·Nucleic Acids Research·Xueping ZhangZendra E Zehner
Dec 23, 2004·Journal of Virology·Elizabeth A Holley-GuthrieShannon C Kenney
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Aug 28, 2020·Scientific Reports·Zhiyuan V ZouPer Lindahl
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Feb 24, 2009·Biochimica Et Biophysica Acta·Morgan SalmonZendra E Zehner

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