Negative regulation of cyclin-dependent kinase 5 targets by protein kinase C

European Journal of Pharmacology
Bogachan SahinJames A Bibb

Abstract

Cyclin-dependent kinase 5 (Cdk5) is a proline-directed protein serine/threonine kinase essential for brain development and implicated in synaptic plasticity, dopaminergic neurotransmission, drug addiction, and neurodegenerative disorders. Relatively little is known about the molecular mechanisms that regulate the activity of Cdk5 in vivo. In order to determine whether protein kinase C (PKC) regulates Cdk5 activity in the central nervous system, the phosphorylation levels of two Cdk5 substrates were evaluated under conditions of altered PKC activity in vivo. Treatment of acute striatal slices with a PKC-activating phorbol ester caused a time- and dose-dependent decrease in the levels of phospho-Ser6 inhibitor-1, phospho-Ser67 inhibitor-1, and phospho-Thr75 dopamine- and cAMP-regulated phosphoprotein, Mr 32,000 (DARPP-32). This effect was reversed by the PKC inhibitor, Ro-32-0432. Moreover, phospho-Ser6 inhibitor-1, phospho-Ser67 inhibitor-1, and phospho-Thr75 DARPP-32 levels were elevated in brain tissue from mice lacking the gene for PKC-alpha. PKC did not phosphorylate Cdk5 or its cofactor, p25, in vitro. Striatal levels of the Cdk5 cofactor, p35, did not change in response to phorbol ester treatment. Furthermore, Cdk5 immunop...Continue Reading

References

Aug 18, 1991·The Journal of Comparative Neurology·E L GustafsonP Greengard
Nov 10, 1995·The Journal of Biological Chemistry·D TangJ H Wang
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·T OhshimaA B Kulkarni
Oct 6, 1997·Neurochemistry International·S L GreenR Vulliet
Jul 24, 1998·Archives of Biochemistry and Biophysics·R Ricciarelli, A Azzi
Oct 9, 1998·The Journal of Biological Chemistry·J H ConnorS Shenolikar
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·P SharmaH C Pant
Apr 5, 2000·The Journal of Biological Chemistry·G KusakawaS Hisanaga
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·A NishiP Greengard
Mar 30, 2001·The Journal of Biological Chemistry·J A BibbP Greengard
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·F LiuP Greengard
Oct 5, 2001·Nature Reviews. Molecular Cell Biology·R Dhavan, L H Tsai
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·B S LiH C Pant
Jan 11, 2002·The Journal of Biological Chemistry·Holger Patzke, Li-Huei Tsai
Sep 13, 2002·The Journal of Biological Chemistry·Mitsuko HashiguchiToshio Hashiguchi
Oct 24, 2002·Brain Research. Molecular Brain Research·Petri KerokoskiTuula Pirttilä
Jul 12, 2003·Nature Cell Biology·Timothy C TanPhillip J Robinson
Dec 16, 2003·Neuro-Signals·Anthony C B LimRobert Z Qi
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sang Yoon LeePietro De Camilli
Jan 30, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Maria A MorabitoLi-Huei Tsai
Feb 18, 2004·Nature Medicine·Julian C BrazJeffery D Molkentin
Apr 9, 2005·Journal of Neurochemistry·Fan-Yan WeiShin-Ichi Hisanaga
Apr 3, 2007·The Journal of Biological Chemistry·Chan NguyenJames A Bibb

❮ Previous
Next ❯

Citations

Nov 16, 2013·Journal of Molecular Neuroscience : MN·Zheng WenBo Tian
Nov 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ivan O MedvedevAli Salahpour
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chun-tao ZhaoXiao-bing Yuan
Feb 12, 2015·British Journal of Pharmacology·Jean-Martin BeaulieuRaul R Gainetdinov
Jul 17, 2009·American Journal of Physiology. Cell Physiology·Valeria PadovanoGrazia Pietrini
Nov 18, 2018·Cellular and Molecular Neurobiology·Marianne O KleinRicardo G Correa
Jul 25, 2013·Journal of Proteome Research·Li XiaGuo-Qiang Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
David R BenavidesJames A Bibb
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Tamy C Frank-CannonMalú G Tansey
© 2021 Meta ULC. All rights reserved