Negative regulation of the major human AP-endonuclease, a multifunctional protein

Biochemistry
T IzumiS Mitra

Abstract

Abasic sites in DNA are generated either spontaneously or after removal of altered bases during the base excision repair process. These as well as 3' damaged ends of DNA at single-strand breaks induced by reactive oxygen species are repaired by AP-endonucleases. The major human AP-endonuclease (named APE-1) has two unrelated activities. It may function as an activator of c-Fos and c-Jun transcription factors and as a repressor of the parathyroid hormone (PTH) gene by binding to the negative Ca(2+)-response elements (nCaRE) in its promoter. Preliminary studies indicate that the h-APE-1 gene is highly regulated. Analysis of its promoter activity by transient expression of the luciferase reporter gene in human, HeLa and TK6 cells suggested the presence of a negative regulatory element in the promoter. Two nCaRE-like sequences were identified in the promoter segment responsible for inhibiting reporter gene expression. Competitive electrophoretic mobility shift assay with HeLa nuclear extract indicated that the nCaRE sequences of the APE-1 and PTH genes are recognized by the APE-1 polypeptide. These results suggest that the APE-1 gene may be down-regulated by its own product.

References

Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Jan 1, 1994·Annual Review of Biochemistry·B Demple, L Harrison
Nov 1, 1994·International Journal of Radiation Biology·S S Wallace

❮ Previous
Next ❯

Citations

Nov 6, 2003·Toxicology·Gerhard FritzBernd Kaina
Nov 21, 2000·The International Journal of Biochemistry & Cell Biology·G Fritz
Nov 21, 2007·The Journal of Experimental Medicine·Jeroen E J GuikemaCarol E Schrader
Feb 12, 2005·Antioxidants & Redox Signaling·Gianluca TellMark R Kelley
Nov 4, 2008·Antioxidants & Redox Signaling·Gianluca TellMark R Kelley
Jun 9, 2005·Nucleic Acids Research·Elias B JacksonTadahide Izumi
Jul 6, 2011·Nucleic Acids Research·Carlos S BussoTadahide Izumi
Dec 4, 2012·International Journal of Molecular Sciences·Judith-Irina Pagel, Elisabeth Deindl
Aug 12, 1999·Biochemical and Biophysical Research Communications·S Grösch, B Kaina
Dec 21, 2013·Molecular Biology of the Cell·Giulia AntonialiGianluca Tell
Jul 10, 2013·Antioxidants & Redox Signaling·Mengxia Li, David M Wilson
Apr 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tadahide IzumiSankar Mitra
Oct 6, 2000·Mutation Research·A R EvansM R Kelley
Aug 14, 2010·Cellular and Molecular Life Sciences : CMLS·Gianluca TellFranco Quadrifoglio
Dec 14, 2011·Mutation Research·Valeria SimonelliEugenia Dogliotti
Jun 15, 2007·Molecular Aspects of Medicine·Melissa L Fishel, Mark R Kelley
Jul 20, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sanjay M MallyaAndrew Arnold
Nov 16, 2004·Mechanisms of Ageing and Development·Bartosz SzczesnyIstvan Boldogh
Jun 30, 2000·Japanese Journal of Pharmacology·S Y NiuN Miki
Dec 18, 2003·The Journal of Clinical Investigation·Lorne J HofsethCurtis C Harris
May 15, 2013·World Journal of Hepatology·Shinichi SumiyoshiHirotoshi Nakamura
Feb 2, 1999·Neuroreport·M EdwardsJ R Perez-Polo
Oct 23, 2020·DNA Repair·Griffin Wright, Natalie R Gassman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.