PMID: 8944660Nov 1, 1996Paper

Negative regulation of the rat Na-K-ATPase alpha 3-subunit gene promoter by thyroid hormone

The American Journal of Physiology
H HeG Gick

Abstract

Na-K-ATPase alpha 3-subunit mRNA levels are both positively and negatively controlled by thyroid hormone [3,5,3'triiodothyronine (T3)] in primary cultures of neonatal rat cardiac myocytes. In this study, transient transfection analysis indicated that two regions of the rat alpha 3 gene between nucleotides -116 and -6 and -6 and +80 conferred T3-mediated inhibition of reporter gene expression. Electrophoretic mobility shift assays showed specific binding of T3 receptor monomers and T3 receptor-retinoid X receptor heterodimers at each alpha 3 gene negative T3-response region. The alpha 3 gene region from -116 to -6 base pairs also mediates repression in response to retinoic acid (RA) and binds RA receptor. In the absence of ligand, reporter gene expression driven by the -116 to -6-base pair region is repressed with cotransfection of T3 receptor, whereas it is unaffected by overexpression of RA receptor. These data demonstrate that the proximal promoter of the rat Na-K-ATPase alpha 3 gene contains sequence motifs that mediate repression of alpha 3 gene transcription in response to either T3 or RA in neonatal rat cardiac myocytes.

References

Dec 1, 1992·Circulation Research·T KamitaniT Kojima
Jan 1, 1991·The Journal of Membrane Biology·J Melikian, F Ismail-Beigi
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Citations

Aug 17, 1999·The Journal of Clinical Investigation·W M KueblerJ Bhattacharya
Dec 23, 2004·Journal of Cardiovascular Pharmacology·Keiko TakahashiSatsuki Koida
Jun 17, 2008·American Journal of Respiratory and Critical Care Medicine·Maneesh BhargavaDavid H Ingbar
Nov 19, 2015·Frontiers in Cell and Developmental Biology·Zhiqin Li, Sigrid A Langhans
Sep 26, 2000·Molecular Genetics and Metabolism·A Habiba, R W Mercer
Nov 1, 1997·Trends in Cardiovascular Medicine·S M Smith, E D Dickman

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